斯洛文尼亚沿海-喀斯特和沿海-内卡尼奥拉地区蜱类伯氏疏螺旋体的检测、鉴定和基因分型。

IF 1.5 4区 医学 Q3 PARASITOLOGY
Jana Susnjar, Tjasa Cerar Kisek, Katja Strasek Smrdel, Eva Ruzic-Sabljic, Katja Adam, Vladimir Ivovic
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引用次数: 0

摘要

过去30年来,整个欧洲蜱病媒介物种的密度和传播有所增加,导致莱姆病borreliosis病例增加,包括在斯洛文尼亚。本研究旨在分离Borrelia菌株,并通过培养和PCR方法测定2019年在全国两个地区(沿海-喀斯特地区和沿海-内卡尼奥地区)采集的蓖麻依蚊(Linnaeus)成虫中感氏伯氏疏螺旋体和宫氏疏螺旋体的流行率。采用培养法从两地28/559(5%)蜱中分离出伯氏疏螺旋体。另外用实时荧光定量PCR检测培养阴性样本(531/559,即95%)。在155/531 (29.2%)pcr阳性样本中,扩增flaB或glpQ片段并进一步测序以鉴定伯氏疏螺旋体的种类。采用培养和PCR两种方法检测,疏螺旋体在沿海-喀斯特地区的感染率为32.7%,在沿海-内卡尼诺拉地区的感染率为33.0%。对分离的伯氏疏螺旋体进行基因分型,其中17/28(60%)为格里尼伯氏螺旋体Mlg2亚型。在所有蜱类样本中,8/398份(2%)PCR阳性。根据以往的研究,本研究认为该地区蜱虫感染阿夫泽里贝氏杆菌较多,但基因分型结果显示该地区蜱虫数量最多。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection, identification and genotyping of Borrelia spp. in ticks of Coastal-Karst and Littoral-Inner Carniola regions in Slovenia.

The density and spread of tick vector species have increased throughout Europe in the last 30 years, leading to an increase of Lyme borreliosis cases, including in Slovenia. The aim of this study was to isolate Borrelia strains and determine the prevalence of B. burgdorferi sensu lato and B. miyamotoi in adults of Ixodes ricinus (Linnaeus) collected in 2019 in the two regions of the country (Coastal-Karst and Littoral-Inner Carniola) by cultivation and PCR. We isolated B. burgdorferi s.l. by culture method in 28/559 (5%) ticks from both regions. Culture-negative samples (531/559, i.e., 95%) were additionally tested by real-time PCR. In 155/531 (29.2%) PCR-positive samples, a fragment of flaB or glpQ was amplified and further sequenced to identify species of the Borrelia. Using both methods, cultivation and PCR, Borrelia spp. prevalence was 32.7% in the Coastal-Karst region and 33.0% in the Littoral-Inner Carniola region. Genotyping of the Borrelia spp. isolates revealed that 17/28 (60%) were B. garinii subtype Mlg2. Of all tick samples tested for B. miyamotoi 8/398 (2%) were PCR positive. Based on previous studies in these regions, we had expected more ticks to be infected with B. afzelii, but genotyping revealed that B. garinii was the most abundant.

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来源期刊
Folia Parasitologica
Folia Parasitologica 医学-寄生虫学
CiteScore
2.70
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.
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