Ying Hua Pan, Li Jun Gao, Yun Tao Liang, Yan Zhao, Hai Fu Liang, Wei Wei Chen, Xing Hai Yang, Dong Jin Qing, Ju Gao, Hao Wu, Juan Huang, Wei Yong Zhou, Cheng Cui Huang, Gao Xing Dai, Guo Fu Deng
{"title":"OrMKK3对水稻形态和粒径的影响。","authors":"Ying Hua Pan, Li Jun Gao, Yun Tao Liang, Yan Zhao, Hai Fu Liang, Wei Wei Chen, Xing Hai Yang, Dong Jin Qing, Ju Gao, Hao Wu, Juan Huang, Wei Yong Zhou, Cheng Cui Huang, Gao Xing Dai, Guo Fu Deng","doi":"10.1007/s12374-020-09290-2","DOIUrl":null,"url":null,"abstract":"<p><p>Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from <i>Oryza officinalis</i> Wall. ex Watt encoded by <i>OrMKK3</i>. Different alternative splicing variants occurred in <i>OrMKK3</i>. Green fluorescent protein (GFP)-<i>OrMKK3</i> fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of <i>OrMKK3</i> influenced the expression levels of the grain size-related genes <i>SMG1</i>, <i>GW8</i>, <i>GL3</i>, <i>GW2</i>, and <i>DEP3.</i> Phylogenetic analysis showed that <i>OrMKK3</i> is well conserved in plants while showing large amounts of variation between <i>indica</i>, <i>japonica</i>, and wild rice. In addition, <i>OrMKK3</i> slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, <i>OrMKK3</i>, influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12374-020-09290-2.</p>","PeriodicalId":16762,"journal":{"name":"Journal of Plant Biology","volume":"66 3","pages":"269-282"},"PeriodicalIF":2.2000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12374-020-09290-2","citationCount":"8","resultStr":"{\"title\":\"<i>OrMKK3</i> Influences Morphology and Grain Size in Rice.\",\"authors\":\"Ying Hua Pan, Li Jun Gao, Yun Tao Liang, Yan Zhao, Hai Fu Liang, Wei Wei Chen, Xing Hai Yang, Dong Jin Qing, Ju Gao, Hao Wu, Juan Huang, Wei Yong Zhou, Cheng Cui Huang, Gao Xing Dai, Guo Fu Deng\",\"doi\":\"10.1007/s12374-020-09290-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from <i>Oryza officinalis</i> Wall. ex Watt encoded by <i>OrMKK3</i>. Different alternative splicing variants occurred in <i>OrMKK3</i>. Green fluorescent protein (GFP)-<i>OrMKK3</i> fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of <i>OrMKK3</i> influenced the expression levels of the grain size-related genes <i>SMG1</i>, <i>GW8</i>, <i>GL3</i>, <i>GW2</i>, and <i>DEP3.</i> Phylogenetic analysis showed that <i>OrMKK3</i> is well conserved in plants while showing large amounts of variation between <i>indica</i>, <i>japonica</i>, and wild rice. In addition, <i>OrMKK3</i> slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, <i>OrMKK3</i>, influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12374-020-09290-2.</p>\",\"PeriodicalId\":16762,\"journal\":{\"name\":\"Journal of Plant Biology\",\"volume\":\"66 3\",\"pages\":\"269-282\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s12374-020-09290-2\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12374-020-09290-2\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12374-020-09290-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/4 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
OrMKK3 Influences Morphology and Grain Size in Rice.
Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from Oryza officinalis Wall. ex Watt encoded by OrMKK3. Different alternative splicing variants occurred in OrMKK3. Green fluorescent protein (GFP)-OrMKK3 fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of OrMKK3 influenced the expression levels of the grain size-related genes SMG1, GW8, GL3, GW2, and DEP3. Phylogenetic analysis showed that OrMKK3 is well conserved in plants while showing large amounts of variation between indica, japonica, and wild rice. In addition, OrMKK3 slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, OrMKK3, influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.
Supplementary information: The online version contains supplementary material available at 10.1007/s12374-020-09290-2.
期刊介绍:
Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants.
The Journal publishes the following categories of paper:
Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged.
Reviews -- Invited by the EiC.
Brief Communications -- Concise but independent report representing significant contribution to plant science.
The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.