西班牙马德里某三级医院生殖支原体大环内酯类药物和氟喹诺酮类药物耐药相关突变的流行病学和流行率

IF 1.9 4区医学 Q4 MICROBIOLOGY

Revista Espanola De Quimioterapia Pub Date : 2023-06-01 DOI:10.37201/req/123.2022

A Maldonado-Barrueco, M Rodríguez-Ayala, D Grandioso-Vas, P García-Clemente, G V Guedez-López, J García-Rodríguez, I Quiles-Melero

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引用次数: 0

摘要

目的:生殖支原体引起持续性性传播感染。本研究的目的是估计拉巴斯大学医院(西班牙马德里)患者生殖支原体和性传播合并感染患者对大环内酯类药物和氟喹诺酮类药物耐药的流行情况。方法:对2021年1月至10月就诊的患者进行研究。采用实时荧光定量PCR技术(Allplex,SeegeneTM)筛选性传播病原体，检测23S rRNA和parC基因突变。结果:共研究了1518名女性和1136名男性。生殖支原体患病率为2.1%。大环内酯类药物耐药率为51.8%。发现的突变分别是A2059G、A2058T和A2058G。氟喹诺酮类药物耐药率为17.8%，以G248T突变(S83I)最为常见。7名男性有一些性传播合并感染。结论:虽然生殖支原体感染率较低，但对大环内酯类药物的高耐药率使得性传播感染的诊断和经验治疗方案有必要进行修订。筛选大环内酯类耐药概况后，适合使用氟喹诺酮类药物。

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Epidemiology and prevalence of mutations associated with resistance to macrolides and fluoroquinolones in Mycoplasma genitalium in a tertiary hospital from Madrid, Spain.

Objective: Mycoplasma genitalium causes persistent sexually transmitted infections. The aims of this study were to estimate the prevalence of resistances to macrolides and fluoroquinolones in M. genitalium and the sexually transmitted coinfections in patients at Hospital Universitario La Paz (Madrid, Spain).

Methods: Patients attended between January and October 2021 were studied. Screening for sexually transmitted pathogens and detection of 23S rRNA and parC genes mutations were performed by real-time PCR (Allplex,SeegeneTM).

Results: A total of 1,518 females and 1,136 males were studied. The prevalence of M. genitalium was 2.1%. The macrolides resistance rate was 51.8%. The mutations found were A2059G, A2058T and A2058G. The rate of resistance to fluoroquinolones was 17.8% being the G248T mutation (S83I) the most frequent. Seven males had some sexual transmitted coinfection.

Conclusions: Although the percentage of M. genitalium infections is low, the high rate of resistance to macrolides makes it necessary to revise the protocols for diagnosis and empirical treatment of sexually transmitted infections. The use of fluoroquinolones is appropriate after screening of macrolide resistance profile.

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来源期刊

Revista Espanola De Quimioterapia 医学-药学

CiteScore

2.90

自引率

10.50%

发文量

146

审稿时长

>12 weeks

期刊介绍： The official journal of the Sociedad Española de Quimioterapia (Spanish Society of Chemotherapy), publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents primarily in human medicine. Authors sign an exclusive license agreement, where authors have copyright but license exclusive rights in their article to the Publisher. All manuscripts are free open access. Revista Española de Quimioterapia includes the following sections: reviews, original articles, brierf reports, letters, and consensus documents.