Hologic®Panther AptimaTM严重急性呼吸系统综合征冠状病毒2型检测在检测严重急性呼吸综合征冠状病毒2中的作用:筛查还是诊断技术?

IF 1.9 4区 医学 Q4 MICROBIOLOGY
Revista Espanola De Quimioterapia Pub Date : 2023-10-01 Epub Date: 2023-06-02 DOI:10.37201/req/135.2022
C García-Salguero, L Vallejo, M Martínez-Rodríguez, A Delgado-Iribarren, E Culebras
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引用次数: 0

摘要

在新冠肺炎在世界各地爆发的多波疫情中,进行快速灵敏的诊断检测已成为微生物实验室的优先事项。在Panther系统(Hologic)上运行的AptimaTM严重急性呼吸系统综合征冠状病毒2型转录介导扩增(TMA)检测是满足这一需求的一个非常好的选择。为了评估该系统,研究中包括570个呼吸样本,并通过Panther(Hologic)系统和qRT-PCR(Thermo Fisher Science,Waltham,USA)进行处理,qRT-PCR是目前诊断严重急性呼吸综合征冠状病毒2型(严重急性呼吸系统综合征冠状病毒-2)的检测方法。Panther系统(Hologic)获得了大量假阳性(n=76),但假阳性的数量随着相对光单位(RLU)值的增加而减少。这些结果表明,这种技术可以作为样本筛查的一个很好的选择,但检查阳性结果应该是强制性的,尤其是那些RLU值较低的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Hologic® Panther AptimaTM SARS-CoV-2 assay in the detection of SARS-CoV-2: screening or diagnostic technique?

During the multiple waves of COVID-19 suffered all over the world, having a rapid and sensitive diagnostic test has become a priority for microbiology laboratories. The AptimaTM SARS-CoV-2 transcription-mediated amplification (TMA) assay running on the Panther system (Hologic) was presented as a very good option to cover this need. To evaluate this system, 570 respiratory samples were included in the study and were processed both by the Panther (Hologic) system and by qRT-PCR (Thermo Fisher Science, Waltham, USA), current assay for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A high number of false positives (n=76) was obtained with Panther system (Hologic), but the number of false positives decreases as the relative light units (RLU) value increases. These results show that this technique can be a good option for sample screening but checking for positive results should be mandatory, especially those with low RLU values.

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来源期刊
CiteScore
2.90
自引率
10.50%
发文量
146
审稿时长
>12 weeks
期刊介绍: The official journal of the Sociedad Española de Quimioterapia (Spanish Society of Chemotherapy), publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents primarily in human medicine. Authors sign an exclusive license agreement, where authors have copyright but license exclusive rights in their article to the Publisher. All manuscripts are free open access. Revista Española de Quimioterapia includes the following sections: reviews, original articles, brierf reports, letters, and consensus documents.
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