玉米ZmSWEET15a基因的鉴定与功能分析

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mengtong Liu, Tongyu Liu, Jianyu Lu, Yangyang Zhou, Shubo Liu, Peng Jiao, Siyan Liu, Jing Qu, Shuyan Guan, Yiyong Ma
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引用次数: 2

摘要

糖最终输出转运蛋白(SWEETs)基因家族是一类新型糖转运蛋白,在植物生长发育、生理代谢和非生物胁迫等方面起着重要作用。本研究采用实时荧光定量PCR技术分析了ZmSWEET15a基因在玉米不同器官和不同非生物胁迫下的表达情况。结果表明,ZmSWEET15a在根、茎、叶和籽粒中均有表达,其中在叶片中的表达量最高,与叶片发育高度相关。在聚乙二醇(PEG)、NaCl、H2O2和脱落酸胁迫下,ZmSWEET15a的表达上调,而在冷胁迫下,ZmSWEET15a的表达受到抑制。在糖特异性实验中,我们发现蔗糖是玉米种子萌发最有效的碳源。ZmSWEET15a在不同碳源中的表达分析表明,ZmSWEET15a的表达更容易受到蔗糖的诱导。ZmSWEET15a在玉米植株中过表达可降低叶片中蔗糖含量,提高籽粒中蔗糖含量。ZmSWEET15a在酵母突变株SUSY7/ura中的异源表达表明,ZmSWEET15a是一种蔗糖转运蛋白,与pH无关。该研究为玉米和其他作物的糖转运和碳水化合物分配提供了新的认识,并为在分子水平上提高作物品质提供了更多的遗传信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterization and Functional Analysis of <i>ZmSWEET15a</i> in Maize.

Characterization and Functional Analysis of ZmSWEET15a in Maize.

The sugars will eventually be exported transporters (SWEETs) gene family is a new type of sugar transporters, which plays an important role in plant growth and development, physiological metabolism, and abiotic stress. In this study, we used quantitative real-time PCR to analyze the expression of ZmSWEET15a gene in different organs of maize and under different abiotic stresses. The results showed that ZmSWEET15a was expressed in roots, stems, leaves, and grains, with the highest expression level in leaves, which was highly correlated with leaf development. Under the treatment of polyethylene glycol (PEG), NaCl, H2O2, and abscisic acid stress, the expression of ZmSWEET15a was upregulated, while under the treatment of cold stress, the expression of ZmSWEET15a was inhibited. In sugar-specific experiments, we found that sucrose was the most effective carbon source for maize seed germination. The expression analysis of ZmSWEET15a in different carbon sources suggested that the expression of ZmSWEET15a was more likely to be induced by sucrose. Overexpression of ZmSWEET15a in maize plants could reduce the sucrose content in leaves and increase the sucrose content in grains. The heterologous expression of ZmSWEET15a in the yeast mutant strain SUSY7/ura indicated that ZmSWEET15a is a sucrose transporter and pH independent. This study provides new insight into sugar transport and carbohydrate partitioning in maize and other crops, and provide more genetic information for improving crop quality at the molecular level.

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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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