I Gde Rurus Suryawan, Andrianto, Arifta Devi Anggaraeni, Arisya Agita, Ricardo Adrian Nugraha
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Platelet-rich plasma (PRP), which contains rich growth factors, is believed to stimulate stem cell proliferation and differentiation in the context of cardiac tissue regeneration.</p><p><strong>Objective: </strong>This study aimed to analyze the effect of PRP administration to enhance the differentiation of AMSCs into cardiomyocytes.</p><p><strong>Methods: </strong>This study used a randomized post-test-only controlled group design. AMSCs were isolated from adipose tissues and cultured for 4 passages. The samples were divided into 3 groups, a negative control group (α-MEM), a positive control group (differentiation medium), and a treatment group (PRP). The assessment of GATA-4 expression was conducted using flow cytometry on day-5. The assessment of troponin expression was conducted using immunocytochemistry on day- 10. Data analysis was conducted using T-test and One-Way ANOVA.</p><p><strong>Results: </strong>Flowcytometry of GATA-4 expression revealed a significant improvement in PRP group compared to negative and positive control group (67.04 ± 4.49 vs. 58.15 ± 1.23 p < 0.05; 67.04 ± 4.49 vs. 52.96 ± 2.02 p < 0.05). This was supported by the results of immunocytochemistry on troponin expression, which revealed significant improvement in the PRP group compared to negative and positive controls (38.13 ± 5.2 vs. 10.73 ± 2.39 p < 0.05; 38.13 ± 5.2 vs. 26.00 ± 0.4 p < 0.05).</p><p><strong>Conclusion: </strong>PRP administration in the AMSCs culture could significantly improve the differentiation of AMSCs into cardiomyocytes measured by GATA-4 and troponin expressions. This was concordant with our hypothesis, which stated that there was an effect of PRP administration on AMSCs differentiation into cardiomyocytes.</p>","PeriodicalId":9535,"journal":{"name":"Cardiovascular and Hematological Agents in Medicinal Chemistry","volume":"21 3","pages":"156-166"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Role of Human Platelet-rich Plasma to Enhance the Differentiation of Adipose-derived Mesenchymal Stem Cells into Cardiomyocyte: An Experimental Study.\",\"authors\":\"I Gde Rurus Suryawan, Andrianto, Arifta Devi Anggaraeni, Arisya Agita, Ricardo Adrian Nugraha\",\"doi\":\"10.2174/1871525720666220510211116\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Several studies have shown that adipose-derived mesenchymal stem cells (AMSCs) can differentiate into mesenchymal lineages, including cardiac cell types, but complete differentiation into cardiomyocytes is challenging. 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引用次数: 0
摘要
背景:一些研究表明，脂肪来源的间充质干细胞(AMSCs)可以分化为间充质细胞系，包括心脏细胞类型，但完全分化为心肌细胞是具有挑战性的。不幸的是，最大化AMSCs分化的最佳方法尚未建立。富血小板血浆(PRP)含有丰富的生长因子，被认为在心脏组织再生的背景下刺激干细胞增殖和分化。目的:探讨PRP对AMSCs向心肌细胞分化的促进作用。方法:本研究采用随机后验对照组设计。从脂肪组织中分离出AMSCs，培养4代。将样品分为阴性对照组(α-MEM)、阳性对照组(分化培养基)和处理组(PRP) 3组。第5天采用流式细胞术检测GATA-4表达情况。第10天免疫细胞化学法检测肌钙蛋白表达。数据分析采用t检验和单因素方差分析。结果:流式细胞术检测结果显示，PRP组GATA-4表达水平明显高于阴性和阳性对照组(67.04±4.49∶58.15±1.23 p < 0.05);67.04±4.49 vs. 52.96±2.02 p < 0.05)。免疫细胞化学对肌钙蛋白表达的结果也支持了这一点，PRP组与阴性和阳性对照组相比有显著改善(38.13±5.2比10.73±2.39 p < 0.05;38.13±5.2 vs. 26.00±0.4 p < 0.05)。结论:经GATA-4和肌钙蛋白表达测定，PRP能显著促进AMSCs向心肌细胞的分化。这与我们的假设一致，即PRP给药对AMSCs向心肌细胞分化有影响。
The Role of Human Platelet-rich Plasma to Enhance the Differentiation of Adipose-derived Mesenchymal Stem Cells into Cardiomyocyte: An Experimental Study.
Background: Several studies have shown that adipose-derived mesenchymal stem cells (AMSCs) can differentiate into mesenchymal lineages, including cardiac cell types, but complete differentiation into cardiomyocytes is challenging. Unfortunately, the optimal method to maximize AMSCs differentiation has not yet been established. Platelet-rich plasma (PRP), which contains rich growth factors, is believed to stimulate stem cell proliferation and differentiation in the context of cardiac tissue regeneration.
Objective: This study aimed to analyze the effect of PRP administration to enhance the differentiation of AMSCs into cardiomyocytes.
Methods: This study used a randomized post-test-only controlled group design. AMSCs were isolated from adipose tissues and cultured for 4 passages. The samples were divided into 3 groups, a negative control group (α-MEM), a positive control group (differentiation medium), and a treatment group (PRP). The assessment of GATA-4 expression was conducted using flow cytometry on day-5. The assessment of troponin expression was conducted using immunocytochemistry on day- 10. Data analysis was conducted using T-test and One-Way ANOVA.
Results: Flowcytometry of GATA-4 expression revealed a significant improvement in PRP group compared to negative and positive control group (67.04 ± 4.49 vs. 58.15 ± 1.23 p < 0.05; 67.04 ± 4.49 vs. 52.96 ± 2.02 p < 0.05). This was supported by the results of immunocytochemistry on troponin expression, which revealed significant improvement in the PRP group compared to negative and positive controls (38.13 ± 5.2 vs. 10.73 ± 2.39 p < 0.05; 38.13 ± 5.2 vs. 26.00 ± 0.4 p < 0.05).
Conclusion: PRP administration in the AMSCs culture could significantly improve the differentiation of AMSCs into cardiomyocytes measured by GATA-4 and troponin expressions. This was concordant with our hypothesis, which stated that there was an effect of PRP administration on AMSCs differentiation into cardiomyocytes.
期刊介绍：
Cardiovascular & Hematological Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new Cardiovascular & Hematological Agents. Each issue contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in Cardiovascular & Hematological medicinal chemistry. Cardiovascular & Hematological Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cardiovascular & hematological drug discovery.
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