Suene Moçato Siguematsu Abrão, Danielle Gregorio, Monalisa Kethleen Costa De Azevedo, Graziela Garrido Mori, Regina Célia Poli-Frederico, Luciana Prado Maia
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引用次数: 0
摘要
目的是评估Bio-C Repair (BCR)的体外细胞毒性和遗传毒性，并与Endosequence BC Root Repair (ERRM)、MTA Angelus (MTA- ang)和MTA Repair HP (MTA-HP)进行比较。将MC3T3成骨细胞暴露于修复生物陶瓷水泥提取物中。1、3、7 d后，分别用MTT和微核试验评价细胞毒性和遗传毒性。未暴露于生物材料的细胞作为阴性对照。数据比较采用双因素方差分析，随后采用Tukey检验(α=5%)。在任何实验时间，MTA-Ang和MTA-HP在细胞毒性方面与对照组没有差异。BCR和ERRM在3天和7天后降低了细胞活力(p
Cytotoxicity and genotoxicity of Bio-C Repair, Endosequence BC Root Repair, MTA Angelus and MTA Repair HP.
The aim was to evaluate in vitro cytotoxicity and genotoxicity of Bio-C Repair (BCR), compared to Endosequence BC Root Repair (ERRM), MTA Angelus (MTA-Ang), and MTA Repair HP (MTA-HP). MC3T3 osteoblastic cells were exposed to extracts of the repairing bioceramic cements. After 1, 3, and 7 days, cytotoxicity and genotoxicity were evaluated by MTT and Micronucleus tests, respectively. Cells not exposed to biomaterials were used as a negative control. Data were compared using ANOVA two-way, followed by the Tukey Test (α=5%). MTA-Ang and MTA-HP showed no difference in relation to control regarding cytotoxicity in any experimental times. BCR and ERRM reduced cell viability after 3 and 7 days (p<0.05); however, the reduction caused by BCR was less than that caused by ERRM. Considering the micronucleus formation, all biomaterials caused an increase after 3 and 7 days (p<0.05), being greater for the BCR and ERRM groups. It can be concluded that BCR is non-cytotoxic in osteoblastic cells, as well as MTA-Ang e MTA Repair HP. BCR and ERRM showed greater genotoxicity than others tested biomaterials.
期刊介绍：
Brazilian Dental Journal, publishes Full-Length Papers, Short Communications and Case Reports, dealing with dentistry or related disciplines and edited six times a year.
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