胍丁胺对小鼠变应性气道炎症模型影响的比较研究。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Mohammed K Еlmahdy, Rania R Abdelaziz, Hoda S Elmahdi, Ghada M Suddеk
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引用次数: 0

摘要

简介:哮喘是一种慢性肺部疾病,损害和收缩气道。本研究评估了胍丁氨酸对卵清蛋白(OVA)诱导的气道变应性炎症的影响。方法:采用腹腔注射OVA致敏法诱导小鼠气道炎症,时间分别为第0、7天;然后从第14天至第16天给小鼠注射标准抗哮喘药物倍氯米松(150µg/kg,吸入)。在第0天腹腔注射胍丁胺(200 mg/kg),然后每天注射,持续16天,然后进行卵细胞激发。测定肺重比、总细胞数和分化细胞数、支气管肺泡灌洗液(BALF)中TNF-α、白细胞介素-5 (IL-5)和IL-13、肺亚硝酸盐/硝酸盐(NO)及氧化参数。采用组织病理学和免疫组织化学染色。结果:胍丁胺(200mg /kg)连续注射16 d可明显减轻气道炎症反应。BALF炎症细胞、TNF-α、IL-5、IL-13、肺NO和丙二醛(MDA)水平随超氧化物歧化酶(SOD)水平升高而显著降低。肥大细胞的组织学和免疫组织化学分析与生化改善平行。结论:最后,本研究表明,agmatine通过减轻细胞因子释放、NO表达和氧化应激来减轻OVA引起的气道变应性炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Agmatine on a mouse model of allergic airway inflammation: A comparative study.

Introduction: Asthma is a chronic lung disease that injures and constricts the airways. This study evaluates the effects of agmatine on ovalbumin (OVA)-induced allergic inflammation of the airways.

Methods: OVA sensitization by intraperitoneal injection was used to induce airway inflammation in mice on days 0 and 7; then the mice were challenged using beclomethasone (150 µg/kg, inhalation), a standard anti-asthmatic drug, from day 14 to day 16. Furthermore, agmatine (200 mg/kg) was intraperitoneally injected on day 0 and then daily for 16 days, followed by OVA challenge. The lung weight ratio, total and differential cell counts, TNF-α, interleukin-5 (IL-5) and IL-13 in bronchoalveolar lavage fluid (BALF), lung nitrite/nitrate (NO), and oxidative parameters were determined. Moreover, histopathological and immunohistochemical staining was employed.

Results: Injection of agmatine (200 mg/kg) for 16 days significantly attenuated inflammation of the airways. The levels of BALF inflammatory cells, TNF-α, IL-5, IL-13, lung NO, and malondialdehyde (MDA), significantly decreased with concomitant elevation of superoxide dismutase (SOD) levels. Histological and immunohistochemical analyses of mast cells paralleled to biochemical improvements.

Conclusion: Finally, this study illustrated that agmatine attenuates the allergic inflammation of airways caused by OVA by mitigating cytokines release, NO expression, and oxidative stress.

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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