Minje Kang, Ji-Hye Jung, Ji-Young Kim, Seok-Ho Hong, Young Her
{"title":"口服益生元对小鼠特应性皮炎的治疗和预防作用。","authors":"Minje Kang, Ji-Hye Jung, Ji-Young Kim, Seok-Ho Hong, Young Her","doi":"10.4168/aair.2023.15.3.303","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Recently, interest is increasing in using prebiotics, which are nutrient ingredients of live microorganism that improve the intestinal environments by promoting the growth of beneficial gut microflora. Although numerous studies have demonstrated the beneficial effects of probiotics on atopic dermatitis (AD) development, few have examined preventive and therapeutic effects of prebiotics on the onset and progression of AD.</p><p><strong>Methods: </strong>In this study, we investigated therapeutic and preventive effect of prebiotics, including β-glucan and inulin, using an oxazolone (OX)-induced AD-like mouse model. Prebiotics were orally administered 2 weeks after the end of sensitization period (therapeutic study) and 3 weeks before the initial sensitization (prevention study). The physiological and histological alterations in the skin and gut of the mice were investigated.</p><p><strong>Results: </strong>In the therapeutic study, the severity of skin lesions and inflammatory responses were effectively reduced after administering β-glucan and inulin, respectively. The expression level of calprotectin was significantly decreased by approximately 2-fold (<i>P</i> < 0.05) in the skin and gut of prebiotics-treated mice compared to the control. In addition, epidermal thickness and the number of infiltrated immune cells were markedly reduced in the dermis of prebiotics-treated mice compared <strike>with</strike> to those in the OX-induced mice (<i>P</i> < 0.05). These findings were same as in the prevention study. Importantly, pre-administration of β-glucan and inulin prevented the progression of AD by promoting the growth of good bacteria in the gut of OX-induced AD mice. However, the co-administration of β-glucan and inulin did not show enhanced preventive effects on these alterations.</p><p><strong>Conclusions: </strong>Prebiotics has a therapeutic effect on AD in OX-induced AD mouse model. Moreover, our study suggests that prebiotics prevents the development of AD and this effect is associated with a change in gut microbiome.</p>","PeriodicalId":7547,"journal":{"name":"Allergy, Asthma & Immunology Research","volume":"15 3","pages":"303-315"},"PeriodicalIF":4.1000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/94/aair-15-303.PMC10186118.pdf","citationCount":"2","resultStr":"{\"title\":\"Therapeutic and Preventive Effect of Orally Administered Prebiotics on Atopic Dermatitis in a Mouse Model.\",\"authors\":\"Minje Kang, Ji-Hye Jung, Ji-Young Kim, Seok-Ho Hong, Young Her\",\"doi\":\"10.4168/aair.2023.15.3.303\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Recently, interest is increasing in using prebiotics, which are nutrient ingredients of live microorganism that improve the intestinal environments by promoting the growth of beneficial gut microflora. Although numerous studies have demonstrated the beneficial effects of probiotics on atopic dermatitis (AD) development, few have examined preventive and therapeutic effects of prebiotics on the onset and progression of AD.</p><p><strong>Methods: </strong>In this study, we investigated therapeutic and preventive effect of prebiotics, including β-glucan and inulin, using an oxazolone (OX)-induced AD-like mouse model. Prebiotics were orally administered 2 weeks after the end of sensitization period (therapeutic study) and 3 weeks before the initial sensitization (prevention study). The physiological and histological alterations in the skin and gut of the mice were investigated.</p><p><strong>Results: </strong>In the therapeutic study, the severity of skin lesions and inflammatory responses were effectively reduced after administering β-glucan and inulin, respectively. The expression level of calprotectin was significantly decreased by approximately 2-fold (<i>P</i> < 0.05) in the skin and gut of prebiotics-treated mice compared to the control. In addition, epidermal thickness and the number of infiltrated immune cells were markedly reduced in the dermis of prebiotics-treated mice compared <strike>with</strike> to those in the OX-induced mice (<i>P</i> < 0.05). These findings were same as in the prevention study. Importantly, pre-administration of β-glucan and inulin prevented the progression of AD by promoting the growth of good bacteria in the gut of OX-induced AD mice. However, the co-administration of β-glucan and inulin did not show enhanced preventive effects on these alterations.</p><p><strong>Conclusions: </strong>Prebiotics has a therapeutic effect on AD in OX-induced AD mouse model. 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Therapeutic and Preventive Effect of Orally Administered Prebiotics on Atopic Dermatitis in a Mouse Model.
Purpose: Recently, interest is increasing in using prebiotics, which are nutrient ingredients of live microorganism that improve the intestinal environments by promoting the growth of beneficial gut microflora. Although numerous studies have demonstrated the beneficial effects of probiotics on atopic dermatitis (AD) development, few have examined preventive and therapeutic effects of prebiotics on the onset and progression of AD.
Methods: In this study, we investigated therapeutic and preventive effect of prebiotics, including β-glucan and inulin, using an oxazolone (OX)-induced AD-like mouse model. Prebiotics were orally administered 2 weeks after the end of sensitization period (therapeutic study) and 3 weeks before the initial sensitization (prevention study). The physiological and histological alterations in the skin and gut of the mice were investigated.
Results: In the therapeutic study, the severity of skin lesions and inflammatory responses were effectively reduced after administering β-glucan and inulin, respectively. The expression level of calprotectin was significantly decreased by approximately 2-fold (P < 0.05) in the skin and gut of prebiotics-treated mice compared to the control. In addition, epidermal thickness and the number of infiltrated immune cells were markedly reduced in the dermis of prebiotics-treated mice compared with to those in the OX-induced mice (P < 0.05). These findings were same as in the prevention study. Importantly, pre-administration of β-glucan and inulin prevented the progression of AD by promoting the growth of good bacteria in the gut of OX-induced AD mice. However, the co-administration of β-glucan and inulin did not show enhanced preventive effects on these alterations.
Conclusions: Prebiotics has a therapeutic effect on AD in OX-induced AD mouse model. Moreover, our study suggests that prebiotics prevents the development of AD and this effect is associated with a change in gut microbiome.
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The journal features cutting-edge original research, brief communications, and state-of-the-art reviews in the specialties of allergy, asthma, and immunology, including clinical and experimental studies and instructive case reports. Contemporary reviews summarize information on topics for researchers and physicians in the fields of allergy and immunology. As of January 2017, AAIR do not accept case reports. However, if it is a clinically important case, authors can submit it in the form of letter to the Editor. Editorials and letters to the Editor explore controversial issues and encourage further discussion among physicians dealing with allergy, immunology, pediatric respirology, and related medical fields. AAIR also features topics in practice and management and recent advances in equipment and techniques for clinicians concerned with clinical manifestations of allergies and pediatric respiratory diseases.
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