木拨鼠肝细胞癌冷冻消融的影像学和病理学评价。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2023-04-01 Epub Date: 2023-03-13 DOI:10.30802/AALAS-CM-22-000092
Juan A Esparza-Trujillo, William F Pritchard, Michal Mauda-Havakuk, Matthew F Starost, Paul Wakim, Johnathan Zeng, Andrew S Mikhail, Ivane Bakhutashvili, Bradford J Wood, John W Karanian
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引用次数: 0

摘要

我们将冷冻消融作为一种临床干预方式,用于治疗患有肝细胞癌(HCC)的成年土拨鼠。土拨鼠(n=4)在出生时感染了土拨鼠肝炎病毒,并发展为LI-RADS-5高血管HCC。在21个月大时,他们接受了超声(US)、对比增强CT(CECT)成像和US引导的大部冷冻消融(IcePearl 2.1 CX,Galil,BTG),切除了他们最大的肿瘤(平均HCC体积为49±9 cm³)。冷冻消融使用两个10分钟的冷冻循环进行,每个循环之后是8分钟的解冻循环。第一只土拨鼠在手术后出现严重出血,并被安乐死。在另外3只土拨鼠中,探针轨迹被烧灼,3只都完成了研究。消融术后14天,进行CECT,并对土拨鼠实施安乐死。使用受试者专用的3D打印切割模具对移植的肿瘤进行切片。评估初始肿瘤体积、冷冻消融冰球的大小、大体病理学以及苏木精和伊红染色的组织切片。在US上,固体冰球的边缘具有回声,有密集的声学阴影,平均尺寸为3.1±0.5×2.1±0.4 cm,横截面积为4.7±1.0 cm²。冷冻消融后第14天,3只土拨鼠的CECT显示断流低衰减冷冻损伤,尺寸为2.8±0.3×2.6±0.4×2.93±0.7cm,横截面积为5.8±1.2cm²。组织病理学评估显示出血性坏死,中心无定形凝固性坏死区域被核碎裂碎片包围。大约2.5毫米的凝固性坏死和纤维结缔组织的边缘清楚地将冷冻损伤与邻近的HCC区分开来。肿瘤的部分冷冻消融在14d时产生凝固性坏死,消融边缘清晰。在冷冻消融高血管肿瘤后,烧灼似乎可以防止出血。我们的研究结果表明,患有HCC的土拨鼠可能为研究消融模式和开发新的联合疗法提供一个预测性的临床前模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Imaging and Pathologic Evaluation of Cryoablation of Woodchuck (Marmota Monax) Hepatocellular Carcinoma.

We characterized cryoablation as a mode of clinical intervention in adult woodchucks with hepatocellular carcinoma (HCC). Woodchucks (n = 4) were infected with woodchuck hepatitis virus at birth and developed LI-RADS-5 hypervascular HCC. At 21 mo of age, they underwent ultrasound (US), contrast-enhanced CT (CECT) imaging, and US-guided subtotal cryoablation (IcePearl 2.1 CX, Galil, BTG) of their largest tumor (Mean HCC volume of 49 ± 9 cm³). Cryoablation was performed using two 10-min freeze cycles, each followed by an 8-min thaw cycle. The first woodchuck developed significant hemorrhage after the procedure and was euthanized. In the other 3 woodchucks, the probe track was cauterized and all 3 completed the study. Fourteen days after ablation, CECT was performed, and woodchucks were euthanized. Explanted tumors were sectioned using subject-specific, 3D-printed cutting molds. Initial tumor volume, the size of the cryoablation ice ball, gross pathology and hematoxylin and eosin-stained tissue sections were evaluated. On US, the edges of the solid ice balls were echogenic with dense acoustic shadowing and average dimensions of 3.1 ± 0.5 × 2.1 ± 0.4 cm and cross-sectional area of 4.7 ± 1.0 cm ². On day 14 after cryoablation, CECT of the 3 woodchucks showed devascularized hypo-attenuating cryolesions with dimensions of 2.8 ± 0.3 × 2.6 ± 0.4 × 2.93 ± 0.7 cm and a cross-sectional area of 5.8 ± 1.2 cm². Histopathologic evaluation showed hemorrhagic necrosis with a central amorphous region of coagulative necrosis surrounded by a rim of karyorrhectic debris. A rim of approximately 2.5 mm of coagulative necrosis and fibrous connective tissue clearly demarcated the cryolesion from adjacent HCC. Partial cryoablation of tumors produced coagulative necrosis with well-defined ablation margins at 14 d. Cauterization appeared to prevent hemorrhage after cryoablation of hypervascular tumors. Our findings indicate that woodchucks with HCC may provide a predictive preclinical model for investigating ablative modalities and developing new combination therapies.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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