具有独特融合切割位点基序的禽原avulavirus 1 VG/ ga样分离物的基因组测序和鉴定

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Henry M Kariithi, David L Suarez, James F Davis, Louise Dufour-Zavala, Tim L Olivier, Dawn Williams-Coplin, Abhijeet Bakre, Chang-Won Lee
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引用次数: 0

摘要

采用非靶向新一代测序方法,对从12岁家鸡胴体中采集的口咽拭子样本进行测序,鉴定了禽原avulavirus 1 (AOAV-1) VG/GA样毒株的全基因组序列。该分离物具有一个与低毒力AOAV-1一致的融合(F)蛋白裂解位点基序,但在117位(112G-R-Q-G-R↓F117)有一个独特的基序,这是AOAV-1毒株的典型基序。与其他低毒力病毒相比,在裂解位点上有一个核苷酸的差异,使得分离物可以通过f基因特异性实时逆转录pcr (rRT-PCR)检测到,从而成为一种特异性检测毒力菌株的诊断试验。测定鸡蛋的平均死亡时间和测定鸡的脑内致病性指数,将分离物归为透镜原性。这是美国首次报道在F蛋白裂解位点117位存在苯丙氨酸残基的透镜原性VG/ ga样病毒。除了担心通过切割位点的额外变化可能导致病毒的致病性转移外,我们的发现还需要提高诊断医师对f基因rRT-PCR检测潜在假阳性的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome Sequencing and Characterization of an Avian Orthoavulavirus 1 VG/GA-like Isolate with a Unique Fusion Cleavage Site Motif.

A complete genome sequence of a VG/GA -like strain of avian orthoavulavirus 1 (AOAV-1) was identified by nontargeted next-generation sequencing of an oropharyngeal swab sample collected from a carcass of a 12-mo-old backyard chicken. The isolate has a fusion (F) protein cleavage site motif consistent with a low virulent AOAV-1, but it has a unique motif with phenylalanine at position 117 (112G-R-Q-G-R↓F117), which is typical for virulent AOAV-1 strains. The one nucleotide difference at the cleavage site compared to other low-virulence viruses made the isolate detectable by F-gene-specific real-time reverse transcription-PCR (rRT-PCR) developed as a diagnostic test to specifically detect virulent strains. The mean death time determined in eggs and intracerebral pathogenicity index determined in chickens classified the isolate as lentogenic. This is the first report of a lentogenic VG/GA-like virus with a phenylalanine residue at position 117 of the F protein cleavage site in the United States. In addition to concern for potential pathogenic shift of the virus through additional changes at the cleavage site, our finding warrants increased awareness of diagnosticians of potential false positive F-gene rRT-PCR tests.

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来源期刊
Avian Diseases
Avian Diseases 农林科学-兽医学
CiteScore
2.40
自引率
7.10%
发文量
80
审稿时长
3 months
期刊介绍: Avian Diseases is an international journal dedicated to publishing original basic or clinical research of the highest quality from various disciplines including microbiology, immunology, pathology and epidemiology. Papers on avian diseases relevant to etiology, pathogenesis, diagnosis, treatment, and control are accepted. Manuscripts dealing with avian species other than poultry will be considered only if the subject is relevant to poultry health.
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