Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes
{"title":"裸子形目:胸骨蝶科本征甘菊的细胞遗传学和分子生物学特征:裸子形目性染色体ZW1W2/ZZ系统的首次报道。","authors":"Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes","doi":"10.1089/zeb.2022.0059","DOIUrl":null,"url":null,"abstract":"<p><p>Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving <i>Eigenmannia</i> (Gymnotiformes) fish species, this study aimed to characterize <i>Eigenmannia</i> aff. <i>desantanai</i> of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW<sub>1</sub>W<sub>2</sub>/ZZ, with 2<i>n</i> = 31 for females and 2<i>n</i> = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences <i>COI</i> from the specimens of <i>E</i>. aff. <i>desantanai</i> were obtained, totalizing three haplotypes. The distance <i>p</i> between the haplotypes in <i>E</i>. aff. <i>desantanai</i>, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of <i>E</i>. aff. <i>desantanai</i>. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as <i>E</i>. aff. <i>desantanai</i> may belong to two distinct species.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Cytogenetic and Molecular Characterization of <i>Eigenmannia</i> aff. <i>desantanai</i> (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW<sub>1</sub>W<sub>2</sub>/ZZ in Gymnotiformes.\",\"authors\":\"Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes\",\"doi\":\"10.1089/zeb.2022.0059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving <i>Eigenmannia</i> (Gymnotiformes) fish species, this study aimed to characterize <i>Eigenmannia</i> aff. <i>desantanai</i> of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW<sub>1</sub>W<sub>2</sub>/ZZ, with 2<i>n</i> = 31 for females and 2<i>n</i> = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences <i>COI</i> from the specimens of <i>E</i>. aff. <i>desantanai</i> were obtained, totalizing three haplotypes. The distance <i>p</i> between the haplotypes in <i>E</i>. aff. <i>desantanai</i>, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of <i>E</i>. aff. <i>desantanai</i>. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as <i>E</i>. aff. <i>desantanai</i> may belong to two distinct species.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1089/zeb.2022.0059\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1089/zeb.2022.0059","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Cytogenetic and Molecular Characterization of Eigenmannia aff. desantanai (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW1W2/ZZ in Gymnotiformes.
Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving Eigenmannia (Gymnotiformes) fish species, this study aimed to characterize Eigenmannia aff. desantanai of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW1W2/ZZ, with 2n = 31 for females and 2n = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences COI from the specimens of E. aff. desantanai were obtained, totalizing three haplotypes. The distance p between the haplotypes in E. aff. desantanai, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of E. aff. desantanai. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as E. aff. desantanai may belong to two distinct species.
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Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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