Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes
{"title":"裸子形目:胸骨蝶科本征甘菊的细胞遗传学和分子生物学特征:裸子形目性染色体ZW1W2/ZZ系统的首次报道。","authors":"Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes","doi":"10.1089/zeb.2022.0059","DOIUrl":null,"url":null,"abstract":"<p><p>Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving <i>Eigenmannia</i> (Gymnotiformes) fish species, this study aimed to characterize <i>Eigenmannia</i> aff. <i>desantanai</i> of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW<sub>1</sub>W<sub>2</sub>/ZZ, with 2<i>n</i> = 31 for females and 2<i>n</i> = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences <i>COI</i> from the specimens of <i>E</i>. aff. <i>desantanai</i> were obtained, totalizing three haplotypes. The distance <i>p</i> between the haplotypes in <i>E</i>. aff. <i>desantanai</i>, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of <i>E</i>. aff. <i>desantanai</i>. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as <i>E</i>. aff. <i>desantanai</i> may belong to two distinct species.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"20 2","pages":"77-85"},"PeriodicalIF":1.4000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Cytogenetic and Molecular Characterization of <i>Eigenmannia</i> aff. <i>desantanai</i> (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW<sub>1</sub>W<sub>2</sub>/ZZ in Gymnotiformes.\",\"authors\":\"Laís de Araújo, Laura Ivana Ramos, Margarida Maria de Rossi Vieira, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro, Luciana Andreia Borin-Carvalho, Carlos Alexandre Fernandes\",\"doi\":\"10.1089/zeb.2022.0059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving <i>Eigenmannia</i> (Gymnotiformes) fish species, this study aimed to characterize <i>Eigenmannia</i> aff. <i>desantanai</i> of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW<sub>1</sub>W<sub>2</sub>/ZZ, with 2<i>n</i> = 31 for females and 2<i>n</i> = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences <i>COI</i> from the specimens of <i>E</i>. aff. <i>desantanai</i> were obtained, totalizing three haplotypes. The distance <i>p</i> between the haplotypes in <i>E</i>. aff. <i>desantanai</i>, ranged from 0.2% to 7.1%. 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Cytogenetic and Molecular Characterization of Eigenmannia aff. desantanai (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW1W2/ZZ in Gymnotiformes.
Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving Eigenmannia (Gymnotiformes) fish species, this study aimed to characterize Eigenmannia aff. desantanai of the upper Paraguay River basin through cytogenetic and molecular analyses, to help in the correct identification and delimitation of species. This study reports a multiple sex system of the type ZW1W2/ZZ, with 2n = 31 for females and 2n = 30 for males. A single pair of chromosomes carrying the nucleolar organizing regions (NORs) was detected. The heterochromatin was colocated in NOR sites and mainly located in the centromeric regions of chromosomes. Besides that, individual sequences COI from the specimens of E. aff. desantanai were obtained, totalizing three haplotypes. The distance p between the haplotypes in E. aff. desantanai, ranged from 0.2% to 7.1%. Species delimitation tests indicated the existence of two possible operational taxonomic units of E. aff. desantanai. Thus, this study reports a new multiple sex system in Gymnotiformes and these specimens previously identified as E. aff. desantanai may belong to two distinct species.
期刊介绍：
Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease.
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage.
Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community.
TechnoFish features two types of articles:
TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines
TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community
Zebrafish coverage includes:
Comparative genomics and evolution
Molecular/cellular mechanisms of cell growth
Genetic analysis of embryogenesis and disease
Toxicological and infectious disease models
Models for neurological disorders and aging
New methods, tools, and experimental approaches
Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.
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