DNA低甲基化可能有助于冷冻林蛙大脑的代谢恢复。

IF 2.5 Q3 GENETICS & HEREDITY
Tighe Bloskie, Kenneth B Storey
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引用次数: 0

摘要

转录抑制是极端应激反应的特征,推测在维持低代谢的过程中保存能量资源。近年来,表观遗传调控与许多动物的应激适应密切相关,包括支持林蛙(Rana sylvatica)的抗冻性。然而,在这些多组织分析中经常缺乏神经组织,这是值得调查的。本研究探讨了DNA甲基化的作用,一个核心的表观遗传机制,在木蛙大脑对冷冻的反应。我们使用免疫印迹分析来追踪在冻融循环中森林鼠大脑DNA甲基转移酶(DNMT)、甲基- cpg结合域(MBD)蛋白和TET去甲基化酶的相对表达,包括与冷冻相关亚应激(缺氧和脱水)的选择比较。同时评估了甲基转移酶活性和5-hmC含量。结果表明,冷冻恢复过程中,林蛙大脑DNA低甲基化是通过DNMT3A/3L表达抑制导致DNMT活性降低和TET2/3水平升高导致5-hmC基因组含量升高的共同作用实现的(p < 0.05)。高度脱水期间DNMT1水平升高也值得注意。上述结果表明,缓解转录抑制的5-mC DNA甲基化是木蛙冻融循环的必要组成部分,可能有助于在青蛙解冻和恢复正常代谢活动时恢复正常的转录状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

DNA Hypomethylation May Contribute to Metabolic Recovery of Frozen Wood Frog Brains.

DNA Hypomethylation May Contribute to Metabolic Recovery of Frozen Wood Frog Brains.

DNA Hypomethylation May Contribute to Metabolic Recovery of Frozen Wood Frog Brains.

DNA Hypomethylation May Contribute to Metabolic Recovery of Frozen Wood Frog Brains.

Transcriptional suppression is characteristic of extreme stress responses, speculated to preserve energetic resources in the maintenance of hypometabolism. In recent years, epigenetic regulation has become heavily implicated in stress adaptation of many animals, including supporting freeze tolerance of the wood frog (Rana sylvatica). However, nervous tissues are frequently lacking in these multi-tissue analyses which warrants investigation. The present study examines the role of DNA methylation, a core epigenetic mechanism, in the response of wood frog brains to freezing. We use immunoblot analysis to track the relative expression of DNA methyltransferases (DNMT), methyl-CpG-binding domain (MBD) proteins and ten-eleven-translocation (TET) demethylases across the freeze-thaw cycle in R. sylvatica brain, including selected comparisons to freeze-associated sub-stresses (anoxia and dehydration). Global methyltransferase activities and 5-hmC content were also assessed. The data show coordinated evidence for DNA hypomethylation in wood frog brains during freeze-recovery through the combined roles of depressed DNMT3A/3L expression driving lowered DNMT activity and increased TET2/3 levels leading to elevated 5-hmC genomic content (p < 0.05). Raised levels of DNMT1 during high dehydration were also noteworthy. The above suggest that alleviation of transcriptionally repressive 5-mC DNA methylation is a necessary component of the wood frog freeze-thaw cycle, potentially facilitating the resumption of a normoxic transcriptional state as frogs thaw and resume normal metabolic activities.

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来源期刊
Epigenomes
Epigenomes GENETICS & HEREDITY-
CiteScore
3.80
自引率
0.00%
发文量
38
审稿时长
11 weeks
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