饮食和年龄对雌性C57BL/6J小鼠溃疡性皮炎的影响。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2023-04-01 Epub Date: 2023-03-07 DOI:10.30802/AALAS-CM-22-000096
Alfonso S Gozalo, Patricia M Zerfas, Jing Qin, Derron A Alves, Munir Akkaya, Mirna Y Peña, William R Elkins
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引用次数: 0

摘要

C57BL/6J(B6)小鼠通常受到溃疡性皮炎(UD)的影响,这是一种病因不明的疾病,对治疗反应不佳。为了研究饮食在UD中的可能作用,我们比较了喂食高脂肪饮食的B6雌性小鼠和喂食对照饮食的小鼠的皮肤变化。此外,通过光镜和透射电子显微镜(TEM)检查了无UD、轻度、中度和重度临床症状的小鼠的皮肤样本。与同期喂食对照饮食的小鼠相比,喂食高脂肪饮食2个月的小鼠具有更多的皮肤肥大细胞脱颗粒。无论饮食如何,与年轻小鼠相比,老年小鼠都有更多的皮肤肥大细胞,并且这些细胞中有更多的正在脱颗粒。早期病变的显微镜变化特征为真皮肥大细胞增加,伴有或不伴有角化过度的表皮增生局灶性区域脱颗粒。随着病情的发展,真皮中出现混合但主要是中性粒细胞的炎症细胞浸润,伴有或不伴有表皮侵蚀和结痂形成。透射电镜显示,真皮肥大细胞膜已被破坏并释放出大量电子致密颗粒,而脱颗粒肥大细胞由于颗粒膜的融合而充满了分离和聚结的空隙。溃疡似乎发生得很快,可能是由于肥大细胞颗粒释放的组胺的瘙痒特性引起的剧烈抓挠所致。这项研究表明,雌性B6小鼠的饮食脂肪与皮肤肥大细胞脱颗粒之间存在直接相关性。此外,老年小鼠的皮肤肥大细胞数量和脱颗粒率较高。在UD病例中早期应用旨在预防肥大细胞脱颗粒的治疗可能会产生更好的结果。正如之前使用热量限制的研究所指出的,啮齿动物饮食中较低的脂肪含量可能有助于预防UD。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Contributions of Diet and Age to Ulcerative Dermatitis in Female C57BL/6J Mice.

C57BL/6J (B6) mice are commonly affected by ulcerative dermatitis (UD), a disease of unknown etiology with poor response to treatment. To study the possible role of diet in UD, we compared skin changes in B6 female mice fed a high-fat diet with those of mice fed a control diet. In addition, skin samples from mice with no, mild, moderate, and severe clinical signs of UD were examined by light and transmission electron microscopy (TEM). Mice fed a high-fat diet for 2 mo had more skin mast cell degranulation than did mice fed the control diet for the same period. Regardless of diet, older mice had more skin mast cells and more of these cells were degranulating as compared with younger mice. Microscopic changes in very early lesions were characterized by an increase in dermal mast cells and degranulation with focal areas of epidermal hyperplasia with or without hyperkeratosis. As the condition progressed, a mixed but predominantly neutrophilic inflammatory cell infiltrate appeared in the dermis, with or without epidermal erosion and scab formation. TEM showed that dermal mast cell membranes had disrupted and released of large number of electron-dense granules, whereas degranulated mast cells were filled with isolated and coalescing empty spaces due to fusion of granule membranes. Ulceration appeared to occur very quickly, probably as result of intense scratching due to the pruritogenic properties of the histamine released from mast cell granules. This study showed a direct correlation between dietary fat and skin mast cell degranulation in female B6 mice. In addition, the number of skin mast cells and degranulation rates was higher in older mice. Treatments directed at preventing mast cell degranulation may result in better outcomes when applied early in UD cases. As noted previously in studies using caloric restriction, lower fat content in rodent diets may help prevent UD.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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