海藻酸盐/壳聚糖水凝胶复合材料在脊髓损伤再生中的缓释作用

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Mohammad Amin Jafarimanesh, Jafar Ai, Shahrokh Shojaei, Hossein Ali Khonakdar, Goldis Darbemamieh, Sadegh Shirian
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引用次数: 5

摘要

水凝胶在组织再生和药物输送系统(DDS)中的应用越来越广泛。在这项研究中,我们初步研究了海藻酸盐(Alg)-壳聚糖(Cs) (Alg-Cs)水凝胶(Npch)与人子宫内膜干细胞(hEnSC)的复合包裹丙戊酸(Val)对脊髓损伤(SCI)的再生能力。为了评价合成的水凝胶的稳定性,进行了必要的zeta电位测量。利用傅里叶红外光谱对制备的水凝胶进行了物理化学评价。研究了合成的水凝胶的物理性能,包括降解率、溶胀能力和可调性。以35只Sprague-Dawley大鼠作脊髓损伤实验,评价水凝胶的神经再生能力。在T9-T10区行椎板切除术暴露脊髓,制作半截脊髓损伤模型。将大鼠随机分为Alg-Cs/Npch、Alg-Cs/Npch/hEnSCs、Alg-Cs/Npch/Val、Alg-Cs/Npch/hEnSCs /Val 5组(n = 7),不作任何干预。FTIR光谱显示了Val、Alg-Cs和海藻酸盐的频带频率和归属。制备的Val/Alg-Cs和Alg-Cs纳米颗粒的平均直径分别为187 nm和210 nm。在Alg-Cs中加入Val后,其尺寸减小了约40 nm。随着培养时间的延长,Cs-Npch/Val水凝胶降解速度明显快于Alg-Cs-/Npch/Val水凝胶。与Cs/Npch/Val和Alg-Cs/Npch/Val水凝胶相比,Alg-Cs/Npch/Val水凝胶具有更高的溶胀能力。Cs-Npch/Val水凝胶比Alg-Cs-/Npch/Val水凝胶降解速度快。Alg-Cs/Npch/hEnSCs/Val可再生受损神经纤维,并在组织学上阻止sci诱导的空泡空间。制备的Alg-Cs/Npch/Val可作为牛磺酸类药物的高分子载体,在神经组织工程(NTE)和DDS中作为生物活性底物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sustained release of valproic acid loaded on chitosan nanoparticles within hybrid of alginate/chitosan hydrogel with/without stem cells in regeneration of spinal cord injury.

Hydrogels have been increasingly applied in tissue regeneration and drug delivery systems (DDS). In this study, the capacity of valproic acid (Val) encapsulated within hybrid of alginate (Alg)-chitosan (Cs) (Alg-Cs) hydrogel containing Cs nanoparticle (Npch) with/without human endometrial stem cells (hEnSC) was initially examined for regeneration of spinal cord injury (SCI). To evaluate the stability of the synthesized hydrogels zeta potential necessary measurements were made. Physicochemically, the developed hydrogels were evaluated using Fourier-transform infrared (FTIR) spectroscopy. The physical properties including degradation rate, swelling ability, and tunability of the synthesized hydrogels were studied. To evaluate the nerve regeneration ability of the synthesized hydrogels, 35 Sprague-Dawley rats were undergone SCI. The spinal cords were exposed using laminectomy in T9-T10 area and the hemi-section SCI model was made. The rats were then randomly divided into 5 groups (n = 7) including, Alg-Cs/Npch, Alg-Cs/Npch/hEnSCs, Alg-Cs/Npch/Val, and Alg-Cs/Npch/hEnScs/Val, and the control groups without any intervention. The FTIR spectra showed band frequencies and assignments of Val, Alg-Cs, and alginate. Nanoparticles were formulated with a mean diameter of 187 and 210 nm, for Val/Alg-Cs and Alg-Cs, respectively. The loading of Val into Alg-Cs led to its reduced size by about 40 nm. The Cs-Npch/Val hydrogels degraded faster than the Alg-Cs-/Npch/Val hydrogel specifically in extended time of incubation. A higher swelling capacity of Alg-Cs/Npch hydrogel, compared to Cs/Npch/Val and Alg-Cs/Npch/Val hydrogels, was found. The Cs-Npch/Val hydrogels degraded faster than Alg-Cs-/Npch/Val hydrogel. The Alg-Cs/Npch/hEnSCs/Val could regenerate the damaged nerve fibers and histologically prevent the SCI-induced vacuolization spaces. The prepared Alg-Cs/Npch/Val could be a suitable polymeric carrier for taurine drugs as bioactive substrate in nerve tissue engineering (NTE) and DDS.

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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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