纤维蛋白凝块干扰人绒毛膜促性腺激素测定导致假唐氏综合征筛查结果。

IF 3.8 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Arzu Etem Akagac, Hatice Bozkurt Yavuz
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引用次数: 0

摘要

血清样本一般用于测定人绒毛膜促性腺激素(hCG),以计算孕中期产妇筛查结果。较低的hCG浓度与较低的唐氏综合征(DS)计算风险相关。因此,由于纤维蛋白凝块导致的错误的低hCG结果可能导致误解。我们报告一位怀孕17+3周的23岁女性。血液被带入Becton-Dickinson (BD)真空容器SST-II先进管(参考:367955)。hCG检测在Immulite 2000 XPi分析仪(Siemens Healthcare Diagnostics Inc, Tarrytown, USA)上使用原厂试剂进行。同一样品的结果分别为2566 U/L、18153 U/L和7748 U/L。去除小纤维蛋白凝块再离心后连续3次的结果分别为18,878、20,255和22,339 U/L。hCG浓度为2556 U/L时发生DS和MoM的风险分别< 1/ 10000和0.14。当hCG浓度为20,255 U/L时,这些值分别为1/5632和1.13。实验室专业人员和技术人员应该意识到,由于小纤维蛋白凝块的干扰,使用Immulite 2000 XPi可能会测量出错误的低hCG结果。错误低估hCG值会降低MoM值,从而降低DS的计算风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fibrin clot interference in a human chorionic gonadotrophin assay causing a false Down syndrome screening result.

Fibrin clot interference in a human chorionic gonadotrophin assay causing a false Down syndrome screening result.

Serum samples are generally used for the measurement of human chorionic gonadotrophin (hCG) to calculate second-trimester maternal screening results. Lower hCG concentrations correlate with a lower calculated risk of Down syndrome (DS). Hence, erroneously low hCG results due to fibrin clot may lead to misinterpretation. We present a 23-year-old woman with a pregnancy of 17+3 weeks. Blood was taken into the Becton-Dickinson (BD) vacutainer SST-II Advance tube (Ref: 367955). The hCG test was performed on Immulite 2000 XPi analyser (Siemens Healthcare Diagnostics Inc, Tarrytown, USA) with original reagents. The results of the same sample were found as 2566 U/L, 18,153 U/L, and 7748 U/L. Three consecutive results after removal of the small fibrin clot and recentrifugation were 18,878, 20,255, and 22,339 U/L. The risk of DS and MoM for the concentration of 2556 U/L hCG was < 1/10,000 and 0.14, respectively. For a hCG concentration of 20,255 U/L, these values were 1/5632 and 1.13, respectively. Laboratory professionals and technicians should be aware that erroneously low hCG results can be measured with the Immulite 2000 XPi due to interference from small fibrin clots. Falsely underestimated hCG values reduce the MoM values and thus the calculated risk of DS.

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来源期刊
Biochemia Medica
Biochemia Medica 医学-医学实验技术
CiteScore
5.50
自引率
3.00%
发文量
70
审稿时长
>12 weeks
期刊介绍: Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).
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