角膜神经性疼痛小鼠岛叶皮层神经活动和神经炎症因子的改变

IF 2.4 4区 心理学 Q2 BEHAVIORAL SCIENCES
Rui Xu, Yu-Wen Zhang, Qing Gu, Tian-Jie Yuan, Bing-Qian Fan, Jun-Ming Xia, Jin-Hong Wu, Ying Xia, Wen-Xian Li, Yuan Han
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引用次数: 2

摘要

干眼病(DED)影响全球近55%的人;一些研究表明,中枢致敏和神经炎症可能有助于DED角膜神经性疼痛的发展,但这种贡献的潜在机制仍有待研究。切除眶外泪腺建立干眼模型。采用化学和机械刺激法检测角膜过敏反应,开场法检测焦虑水平。静息状态fMRI是一种功能磁共振成像(rs-fMRI)方法,用于解剖受累的大脑区域。低频波动(ALFF)的振幅决定了大脑的活动。还进行了免疫荧光检测和定量实时聚合酶链反应来进一步验证研究结果。与假手术组相比,干眼组补充体感觉区、次级听觉皮层、颗粒岛皮层、颞叶关联区和外嗅皮质脑区的ALFF信号增加。岛叶皮层ALFF的变化与角膜超敏反应(p < 0.01)、c-Fos (p < 0.001)、脑源性神经营养因子(p < 0.01)、TNF-α、IL-6和IL-1β的增加有关(p < 0.05)。相比之下,干眼症组IL-10水平下降(p < 0.05)。胰岛皮质注射酪氨酸激酶受体B激动剂环曲霉素-B可阻断d诱导的角膜超敏反应和炎症细胞因子上调(p < 0.01),而不影响焦虑水平。我们的研究表明,与角膜神经性疼痛和岛叶皮层神经炎症相关的大脑功能活动可能导致干眼性角膜神经性疼痛。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Alteration of neural activity and neuroinflammatory factors in the insular cortex of mice with corneal neuropathic pain

Alteration of neural activity and neuroinflammatory factors in the insular cortex of mice with corneal neuropathic pain

Dry eye disease (DED) affects nearly 55% of people worldwide; several studies have proposed that central sensitization and neuroinflammation may contribute to the developing corneal neuropathic pain of DED, while the underlying mechanisms of this contribution remain to be investigated. Excision of extra orbital lacrimal glands established the dry eye model. Corneal hypersensitivity was examined through chemical and mechanical stimulation, and open field test measured the anxiety levels. Restingstate fMRI is a method of functional magnetic resonance imaging (rs-fMRI) was performed for anatomical involvement of the brain regions. The amplitude of low-frequency fluctuation (ALFF) determined brain activity. Immunofluorescence testing and Quantitative real-time polymerase chain reaction were also performed to further validate the findings. Compared with the Sham group, ALFF signals in the supplemental somatosensory area, secondary auditory cortex, agranular insular cortex, temporal association areas, and ectorhinal cortex brain areas were increased in the dry eye group. This change of ALFF in the insular cortex was linked with the increment in corneal hypersensitivity (p < 0.01), c-Fos (p < 0.001), brain-derived neurotrophic factor (p < 0.01), TNF-α, IL-6, and IL-1β (p < 0.05). In contrast, IL-10 levels (p < 0.05) decreased in the dry eye group. DED-induced corneal hypersensitivity and upregulation of inflammatory cytokines could be blocked by insular cortex injection of Tyrosine Kinase receptor B agonist cyclotraxin-B (p < 0.01) without affecting anxiety levels. Our study reveals that the functional activity of the brain associated with corneal neuropathic pain and neuroinflammation in the insular cortex might contribute to dry eye-related corneal neuropathic pain.

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来源期刊
Genes Brain and Behavior
Genes Brain and Behavior 医学-行为科学
CiteScore
6.80
自引率
4.00%
发文量
62
审稿时长
4-8 weeks
期刊介绍: Genes, Brain and Behavior was launched in 2002 with the aim of publishing top quality research in behavioral and neural genetics in their broadest sense. The emphasis is on the analysis of the behavioral and neural phenotypes under consideration, the unifying theme being the genetic approach as a tool to increase our understanding of these phenotypes. Genes Brain and Behavior is pleased to offer the following features: 8 issues per year online submissions with first editorial decisions within 3-4 weeks and fast publication at Wiley-Blackwells High visibility through its coverage by PubMed/Medline, Current Contents and other major abstracting and indexing services Inclusion in the Wiley-Blackwell consortial license, extending readership to thousands of international libraries and institutions A large and varied editorial board comprising of international specialists.
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