Jiaju Lu, Ting Lei, Haichuan Yu, Xiaojie Su, Lu Zhang, Yu Zhang
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引用次数: 0
摘要
目的:探讨β1,3- n -乙酰氨基葡萄糖转移酶-3基因(B3GNT3)在食管癌(ESCA)中的作用及机制。方法:采用starBase数据库检测B3GNT3的表达。采用食管鳞癌(ESCC)细胞系KYSE-30和KYSE-410细胞检测B3GNT3功能。采用实时定量聚合酶链反应(qRT-PCR)检测mRNA水平。采用细胞计数试剂盒-8、克隆形成法和transwell法检测细胞增殖、侵袭和迁移的变化。结果:B3GNT3在ESCA组织中的表达高于正常组织。B3GNT3高表达的ESCA患者的总生存率低于B3GNT3低表达的ESCA患者。体外功能实验表明，B3GNT3干扰的KYSE-30和KYSE-410细胞的增殖能力、迁移能力和侵袭能力均低于对照组，而过表达B3GNT3则相反。在ESCC细胞株中沉默B3GNT3表达后，两种细胞株的生长均受到抑制，侵袭性降低。敲低B3GNT3可降低细胞生长速率和Ki-67表达水平。结论:B3GNT3作为癌基因可能促进ESCC细胞的生长、侵袭和迁移。
The role of B3GNT3 as an oncogene in the growth, invasion and migration of esophageal cancer cells.
Purpose: To investigate the role and mechanism of β1,3-N-acetylglucosaminyltransferase-3 gene (B3GNT3) in esophageal cancer (ESCA).
Methods: The starBase database was used to evaluate the expression of B3GNT3. B3GNT3 function was measured using KYSE-30 and KYSE-410 cells of esophageal squamous cell carcinoma (ESCC) cell lines. The mRNA levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8, clone formation assay and transwell assay were used to detect the changes of proliferation, invasion and migration.
Results: B3GNT3 expression was higher in ESCA tissues than in normal tissues. The overall survival rate of ESCA patients with high B3GNT3 expression was lower than that of ESCA patients with low B3GNT3 expression. In vitro functional experiments showed that the proliferation ability, migration and invasion ability of KYSE-30 and KYSE-410 cells with B3GNT3 interference were lower than those of the control, and the overexpression of B3GNT3 had the opposite effect. After silencing B3GNT3 expression in ESCC cell lines, the growth of both cell lines was inhibited and the invasiveness was decreased. Knockdown of B3GNT3 reduced the growth rate and Ki-67 expression level.
Conclusions: B3GNT3, as an oncogene, may promote the growth, invasion and migration of ESCC cell.
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