可注射硫酸纤维素纳米晶水凝胶的细胞浸润和VEGF负载的高效血管生成。

IF 11.3 1区 医学 Q1 Medicine
Kiyoon Min, Giyoong Tae
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引用次数: 1

摘要

背景:植入生物材料支架的细胞浸润和血管生成是成功整合宿主组织和组织再生的关键。纤维素纳米晶体(CNC)是一种纳米级的纤维素衍生物,它可以与盐形成可注射的物理凝胶。硫酸酸化CNC (CNC- s)的硫酸盐基团可以作为多种生长因子和细胞因子的结合域,并具有肝素结合域,使其持续释放。血管内皮生长因子(VEGF)能促进内皮细胞的增殖和血管生成。在这项研究中,负载vegf的CNC-S水凝胶被评价为一种可注射支架,可以诱导细胞浸润和血管生成。方法:将CNC- s水解得到脱硫的CNC (CNC- ds),作为CNC- s的阴性对照组。制备了CNC-S和CNC-DS水凝胶,并对其生物相容性和VEGF释放进行了比较。将载VEGF或不载VEGF的水凝胶皮下注射到小鼠体内,以评估水凝胶的生物相容性、细胞浸润和血管生成诱导。结果:两种水凝胶均具有相近的稳定性和剪切减薄性能,可作为注射用水凝胶。然而,CNC-S水凝胶表现出VEGF的持续释放(直到8周),而CNC-DS表现出VEGF的快速释放和大爆发。与CNC-DS水凝胶相比,皮下注射的CNC-S水凝胶细胞浸润增强,生物相容性更好,异物反应较轻。此外,负载vegf的CNC-S水凝胶在水凝胶内部诱导了显著的血管生成,而负载vegf的CNC-DS则没有。结论:CNC-S具有良好的可注射性、生物相容性、细胞浸润性和生长因子缓释性。负载vegf的CNC-S水凝胶在水凝胶内部表现出高效的血管生成。CNC- s的硫酸盐基团是vegf负载CNC水凝胶具有良好生物相容性和生物活性的关键。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading.

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading.

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading.

Cellular infiltration in an injectable sulfated cellulose nanocrystal hydrogel and efficient angiogenesis by VEGF loading.

Background: Cellular infiltration and angiogenesis into implanted biomaterial scaffolds are crucial for successful host tissue integration and tissue regeneration. Cellulose nanocrystal (CNC) is a nano-sized cellulose derivative, which can form an injectable physical gel with salts. Sulfate groups of sulfated CNC (CNC-S) can act as a binding domain to various growth factors and cytokines with a heparin-binding domain for sustained release of them. Vascular endothelial growth factor (VEGF) can promote the proliferation of endothelial cells and angiogenesis. In this study, VEGF-loaded CNC-S hydrogel was evaluated as an injectable scaffold that can induce cellular infiltration and angiogenesis.

Methods: CNC-S was hydrolyzed to get desulfated CNC (CNC-DS), which was used as a negative control group against CNC-S. Both CNC-S and CNC-DS hydrogels were prepared and compared in terms of biocompatibility and VEGF release. The hydrogels with or without VEGF loading were subcutaneously injected into mice to evaluate the biocompatibility, cellular infiltration, and angiogenesis induction of the hydrogels.

Results: Both hydrogels possessed similar stability and shear-thinning behavior to be applicable as injectable hydrogels. However, CNC-S hydrogel showed sustained release (until 8 weeks) of VEGF whereas CNC-DS showed a very fast release of VEGF with a large burst. Subcutaneously injected CNC-S hydrogel showed much enhanced cellular infiltration as well as better biocompatibility with milder foreign body response than CNC-DS hydrogel. Furthermore, VEGF-loaded CNC-S hydrogel induced significant angiogenesis inside the hydrogel whereas VEGF-loaded CNC-DS did not.

Conclusion: CNC-S possesses good properties as a biomaterial including injectability, biocompatibility, and allowing cellular infiltration and sustained release of growth factors. VEGF-loaded CNC-S hydrogel exhibited efficient angiogenesis inside the hydrogel. The sulfate group of CNC-S was a key for good biocompatibility and the biological activities of VEGF-loaded CNC hydrogel.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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