基于细菌的多重系统通过光热杀伤和保护性免疫激发来根除耐药细菌的复发性感染。

IF 11.3 1区 医学 Q1 Medicine
Youcui Xu, Yi Wu, Yi Hu, Mengran Xu, Yanyan Liu, Yuting Ding, Jing Chen, Xiaowan Huang, Longping Wen, Jiabin Li, Chen Zhu
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引用次数: 2

摘要

背景:耐药细菌感染的高死亡率是一个难以解决的临床问题,原因是耐药细菌对抗生素的敏感性低,且反复感染的发生率高。方法:以天然palustris红假单胞菌(Rhodopseudomonas palustris, Rp)和美国食品药品监督管理局批准的铝(Al)佐剂组成的光合细菌复合体系(Rp@Al),用于抵抗耐药细菌感染并防止其复发。对其光热性能和体外、体内抗菌能力进行了检测;揭示了其保护性免疫调节作用;验证其对反复感染的预防效果;并论证了系统的安全性。结果:Rp@Al具有优异的光热性能,可有效消除耐甲氧西林金黄色葡萄球菌(MRSA)。此外,Rp@Al增强树突状细胞活化,并进一步触发T辅助1 (TH1)/TH2免疫应答,从而产生针对复发性MRSA感染的病原体特异性免疫记忆。在第二次感染时,Rp@Al-treated小鼠在接近致死的感染剂量下表现出明显较低的细菌负担,更快的脓肿恢复,以及比对照小鼠更高的存活率。结论:该创新的复合系统具有良好的光热和免疫调节作用,在耐药细菌感染的治疗和预防方面具有很大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation.

Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation.

Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation.

Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation.

Background: The high mortality associated with drug-resistant bacterial infections is an intractable clinical problem resulting from the low susceptibility of these bacteria to antibiotics and the high incidence of recurrent infections.

Methods: Herein, a photosynthetic bacteria-based multiplex system (Rp@Al) composed of natural Rhodopseudomonas palustris (Rp) and Food and Drug Administration-approved aluminum (Al) adjuvant, was developed to combat drug-resistant bacterial infections and prevent their recurrence. We examined its photothermal performance and in vitro and in vivo antibacterial ability; revealed its protective immunomodulatory effect; verified its preventative effect on recurrent infections; and demonstrated the system's safety.

Results: Rp@Al exhibits excellent photothermal properties with an effective elimination of methicillin-resistant Staphylococcus aureus (MRSA). In addition, Rp@Al enhances dendritic cell activation and further triggers a T helper 1 (TH1)/TH2 immune response, resulting in pathogen-specific immunological memory against recurrent MRSA infection. Upon second infection, Rp@Al-treated mice show significantly lower bacterial burden, faster abscess recovery, and higher survival under near-lethal infection doses than control mice.

Conclusions: This innovative multiplex system, with superior photothermal and immunomodulatory effects, presents great potential for the treatment and prevention of drug-resistant bacterial infections.

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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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