用毒品和犯罪问题办公室的方法评价Δ9-tetrahydrocannabinolic酸和大麻二酚酸的脱羧效率。

IF 2.8 4区 医学 Q2 TOXICOLOGY
Kenji Tsujikawa, Yuki Okada, Hiroki Segawa, Tadashi Yamamuro, Kenji Kuwayama, Tatsuyuki Kanamori, Yuko T Iwata
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引用次数: 1

摘要

目的:通过加热将Δ9-tetrahydrocannabinolic酸(Δ9-THCA)脱羧为Δ9-tetrahydrocannabinol (Δ9-THC)是测定总Δ9-THC的常用方法。在大麻鉴定和分析手册中,联合国毒品和犯罪问题办公室(毒品和犯罪问题办公室)提出了脱羧条件。虽然该手册的主要分析目标是Δ9-THC,但一些报告也量化了大麻二酚(CBD)。作者评估了Δ9-THCA和大麻二酚酸(CBD的羧化形式)在四种脱羧条件下的脱羧效率,包括毒品和犯罪问题办公室的条件。方法:Δ9-THCA和CBDA在2 mL玻璃瓶中150℃加热12 min,处理如下:条件A为不加盖的乙醇,条件B为不加盖的溶剂,条件C为不加盖的溶剂,条件D (UNODC条件)为不加盖的乙醇中加入0.5 mg/mL三苄胺(TBA)。加热后的残渣用甲醇溶解,用高效液相色谱法进行分析。结果:A和b条件下Δ9-THC和CBD的产量较低(≤10.1%),而C条件下Δ9-THC的产量提高了53.4%,CBD的产量几乎没有提高(11.7%)。在D条件下,Δ9-THC和CBD的产量分别显著提高到83.2%和71.0%。结论:TBA促进了Δ9-THC和CBD羧基化产物的生成;然而,即使有TBA的存在,他们的产量也没有达到100%。法医毒理学家应了解毒品和犯罪问题办公室条件下脱羧的有效性和局限性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluation of decarboxylation efficiency of Δ<sup>9</sup>-tetrahydrocannabinolic acid and cannabidiolic acid by UNODC method.

Evaluation of decarboxylation efficiency of Δ9-tetrahydrocannabinolic acid and cannabidiolic acid by UNODC method.

Purpose: Decarboxylation of Δ9-tetrahydrocannabinolic acid (Δ9-THCA) to Δ9-tetrahydrocannabinol (Δ9-THC) by heating is a common method for determining total Δ9-THC. In the manual for cannabis identification and analysis, the United Nations Office on Drugs and Crime (UNODC) proposed decarboxylation conditions. Although the manual's primary analytical target is Δ9-THC, some reports also quantified cannabidiol (CBD). The authors assessed the efficiency of decarboxylation of Δ9-THCA and cannabidiolic acid (CBDA), a carboxylated form of CBD, under four decarboxylation conditions, including the UNODC condition.

Methods: Δ9-THCA and CBDA were heated in 2-mL glass vials at 150 °C for 12 min after the following treatment: condition A involves the addition of ethanol without capping, condition B involves non addition of solvent without capping, condition C involves non addition of solvent with capping, and condition D (UNODC condition) involves the addition of 0.5 mg/mL tribenzylamine (TBA) in ethanol without capping. The residue after heating was dissolved in methanol and then analyzed by high-performance liquid chromatography.

Results: The production of Δ9-THC and CBD was low (≤ 10.1%) under conditions A and B. Under condition C, Δ9-THC production was increased (53.4%), but CBD production was hardly improved (11.7%). Under condition D, Δ9-THC and CBD production dramatically increased to 83.2 and 71.0%, respectively.

Conclusions: These findings indicated that TBA improved the production of Δ9-THC and CBD from their carboxylated forms; however, even in the presence of TBA, their production did not reach 100%. Forensic toxicologists should understand the effectiveness and limitations of decarboxylation under the UNODC condition.

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来源期刊
Forensic Toxicology
Forensic Toxicology TOXICOLOGY-
CiteScore
5.80
自引率
9.10%
发文量
40
审稿时长
3 months
期刊介绍: The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).
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