HDX-MS基本原理。

IF 5.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Essays in biochemistry Pub Date : 2023-03-29 DOI:10.1042/EBC20220111

Vanesa Vinciauskaite, Glenn R Masson

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引用次数: 6

摘要

氢氘交换质谱(HDX-MS)正在成为分子生物学家用于研究蛋白质结构和动力学的标准技术曲目的一部分。这在一定程度上是由于在该技术的各个阶段越来越多地使用自动化及其应用的多功能性——许多对x射线晶体学和低温电子显微镜等技术提出挑战的蛋白质都可以用HDX-MS进行研究。目前的评论是针对那些对这项技术好奇的科学家，以及它如何帮助他们的研究。它描述了溶剂交换的基本基础，标准HDX-MS实验的基础，并强调了新兴的新实验进展，指出了该领域的发展方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fundamentals of HDX-MS.

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Fundamentals of HDX-MS.

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

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来源期刊

Essays in biochemistry 生物-生化与分子生物学

CiteScore

10.50

自引率

0.00%

发文量

105

审稿时长

>12 weeks

期刊介绍： Essays in Biochemistry publishes short, digestible reviews from experts highlighting recent key topics in biochemistry and the molecular biosciences. Written to be accessible for those not yet immersed in the subject, each article is an up-to-date, self-contained summary of the topic. Bridging the gap between the latest research and established textbooks, Essays in Biochemistry will tell you what you need to know to begin exploring the field, as each article includes the top take-home messages as summary points. Each issue of the journal is guest edited by a key opinion leader in the area, and whether you are continuing your studies or moving into a new research area, the Journal gives a complete picture in one place. Essays in Biochemistry is proud to publish Understanding Biochemistry, an essential online resource for post-16 students, teachers and undergraduates. Providing up-to-date overviews of key concepts in biochemistry and the molecular biosciences, the Understanding Biochemistry issues of Essays in Biochemistry are published annually in October.