基于深度学习的图像分析显示,克罗恩病和溃疡性结肠炎患者粘膜CD3和γδ T细胞的数量和分布存在显著差异

IF 3.4 2区 医学 Q1 PATHOLOGY
Elin Synnøve Røyset, Henrik P Sahlin Pettersen, Weili Xu, Anis Larbi, Arne K Sandvik, Sonja E Steigen, Ignacio Catalan-Serra, Ingunn Bakke
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引用次数: 2

摘要

溃疡性结肠炎(UC)和克罗恩病(CD)的结肠黏膜显示免疫细胞的数量和分布的差异,这是难以通过眼睛评估的。基于深度学习的全幻灯片图像分析(wsi)允许提取复杂的定量数据,可用于揭示不同的炎症模式。我们的目的是探讨CD3和γδ T细胞在组织学上不活跃和活跃的炎症性肠病的结肠粘膜腔室中的分布。通过基于深度学习的分割和细胞检测,从明确定义的CD (n = 37), UC (n = 58)和健康对照(hc, n = 33)的wsi中,我们量化了由Nancy组织学指数定义的近端和远端结肠粘膜上皮内和上皮下以及固有层的CD3和γδ T细胞。我们发现,失活的CD细胞上皮内γδ T细胞明显少于失活的UC细胞,但所有区室的CD3细胞总数均高于UC和hc细胞。在UC中,疾病活动性与上皮内γδ T细胞的大量损失相关,而在CD中则不然。无论疾病活动性如何,CD3细胞的上皮内总数量保持不变。结肠近端粘膜CD3和γδ T细胞比远端多。口服皮质类固醇对γδ T细胞数量有影响,而年龄、性别和疾病持续时间没有影响。粘膜和血液中γδ T细胞的相对丰度无相关性。本研究揭示了CD3和γδ T细胞总数在CD、UC和hc之间的显著差异,特别是上皮区域,并证明了深度分割技术在定量粘膜室细胞中的有用应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Deep learning-based image analysis reveals significant differences in the number and distribution of mucosal CD3 and γδ T cells between Crohn's disease and ulcerative colitis

Deep learning-based image analysis reveals significant differences in the number and distribution of mucosal CD3 and γδ T cells between Crohn's disease and ulcerative colitis

Colon mucosae of ulcerative colitis (UC) and Crohn's disease (CD) display differences in the number and distribution of immune cells that are difficult to assess by eye. Deep learning-based analysis on whole slide images (WSIs) allows extraction of complex quantitative data that can be used to uncover different inflammatory patterns. We aimed to explore the distribution of CD3 and γδ T cells in colon mucosal compartments in histologically inactive and active inflammatory bowel disease. By deep learning-based segmentation and cell detection on WSIs from a well-defined cohort of CD (n = 37), UC (n = 58), and healthy controls (HCs, n = 33), we quantified CD3 and γδ T cells within and beneath the epithelium and in lamina propria in proximal and distal colon mucosa, defined by the Nancy histological index. We found that inactive CD had significantly fewer intraepithelial γδ T cells than inactive UC, but higher total number of CD3 cells in all compartments than UC and HCs. Disease activity was associated with a massive loss of intraepithelial γδ T cells in UC, but not in CD. The total intraepithelial number of CD3 cells remained constant regardless of disease activity in both CD and UC. There were more mucosal CD3 and γδ T cells in proximal versus distal colon. Oral corticosteroids had an impact on γδ T cell numbers, while age, gender, and disease duration did not. Relative abundance of γδ T cells in mucosa and blood did not correlate. This study reveals significant differences in the total number of CD3 and γδ T cells in particularly the epithelial area between CD, UC, and HCs, and demonstrates useful application of deep segmentation to quantify cells in mucosal compartments.

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来源期刊
Journal of Pathology Clinical Research
Journal of Pathology Clinical Research Medicine-Pathology and Forensic Medicine
CiteScore
7.40
自引率
2.40%
发文量
47
审稿时长
20 weeks
期刊介绍: The Journal of Pathology: Clinical Research and The Journal of Pathology serve as translational bridges between basic biomedical science and clinical medicine with particular emphasis on, but not restricted to, tissue based studies. The focus of The Journal of Pathology: Clinical Research is the publication of studies that illuminate the clinical relevance of research in the broad area of the study of disease. Appropriately powered and validated studies with novel diagnostic, prognostic and predictive significance, and biomarker discover and validation, will be welcomed. Studies with a predominantly mechanistic basis will be more appropriate for the companion Journal of Pathology.
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