用斑马鱼胚胎进行食品安全评价的氯化钙毒理学。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Ricardo Lacava Bailone, Hirla Costa Silva Fukushima, Luis Kluwe de Aguiar, Ricardo Carneiro Borra
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引用次数: 0

摘要

氯化钙盐(cacl2)在工业上广泛用作食品添加剂;人类的消费水平由国际或政府机构规定。一般来说,食品工业依靠在哺乳动物(如小鼠、大鼠和兔子)身上进行的毒性研究来确定食品安全。然而,在哺乳动物身上进行测试既耗时又昂贵。斑马鱼已被用于一系列毒理学分析,在灵敏度、时间和成本方面具有优势。然而,关于斑马鱼对氯化钙的敏感性是否与用作食品添加剂的氯化钙浓度相当,目前还没有相关信息。本研究的目的是比较斑马鱼胚胎和幼虫对cacl2的耐受性与目前批准的食品添加剂浓度。采用急性毒性、胚胎毒性、心脏毒性和神经毒性试验测定斑马鱼胚胎和幼虫中cacl2的阈值毒性浓度。数据显示,0.4%以上的剂量对发育以及心脏和神经系统的活动有毒性作用。此外,暴露于0.8%和1.6% cacl2的胚胎在24 hpf后全部死亡。这些发现与食品法典委员会批准的氯化钙浓度限值一致。因此,斑马鱼胚胎适合用于食品添加剂的筛选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Calcium Chloride Toxicology for Food Safety Assessment Using Zebrafish (Danio rerio) Embryos.

The salt calcium chloride (CaCl₂) is widely used in industry as a food additive; levels for human consumption are regulated by international or governmental agencies. Generally, the food industry relies on toxicity studies conducted in mammals such as mice, rats, and rabbits for determining food safety. However, testing in mammals is time-consuming and expensive. Zebrafish have been used in a range of toxicological analyses and offer advantages with regard to sensitivity, time, and cost. However, information in not available with regard to whether the sensitivity of zebrafish to CaCl₂ is comparable to the concentrations of CaCl₂ used as food additives. The aim of this study was to compare the CaCl₂ tolerance of zebrafish embryos and larvae with concentrations currently approved as food additives. Acute toxicity, embryotoxicity, cardiotoxicity, and neurotoxicity assays were used to determine the threshold toxic concentration of CaCl₂ in zebrafish embryos and larvae. The data showed that doses above 0.4% had toxic effects on development and on the activity of the cardiac and neuronal systems. Furthermore, all embryos exposed to 0.8 and 1.6% of CaCl₂ died after 24 hpf. These findings are consistent with the limits of CaCl₂ concentrations approved by Codex Alimentarius. Therefore, zebrafish embryos could be suitable for screening food additives.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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