NaB和NaPc通过TLR-2/NF-κB/ROS/NLRP3途径对鲍曼不动杆菌刺激的THP-1细胞的抗炎作用。

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY

Acta Pharmaceutica Pub Date : 2022-12-01 DOI:10.2478/acph-2022-0036

Chen Shu, Zhang Yan-Yan, Zhang Hai, Ding Long-Kun, Xi Yue, Yan Man, Sun Chang, Wu Liang, Hu Hao

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引用次数: 2

摘要

本研究评估了三种主要短链脂肪酸(SCFAs)对鲍曼不动杆菌诱导的THP-1细胞的抗炎作用。3种主要SCFAs均能抑制鲍曼杆菌刺激的THP-1细胞NF-κB通路活性、NLRP3炎性蛋白和GSDMD的表达，增加自噬。三种主要SCFAs，尤其是丁酸钠(NaB)，在THP-1细胞中具有下调ROS和TLR-2表达的作用。NaB和丙酸钠(NaPc)显著抑制IL-1β和IFN-γ的表达，而乙酸钠(NaAc)不显著。结果表明，NaB和NaPc均能显著抑制鲍曼单胞菌诱导的THP-1细胞的炎症反应，且抑制作用顺序为NaB > NaPc > NaAC。NaB和NaPc可能通过TLR-2/NF-κB/ROS/NLRP3信号通路抑制炎症。

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Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway.

Abstract This study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and increase autophagy. The three main SCFAs, especially the sodium butyrate (NaB), had the effect of down-regulation of ROS and TLR-2 expression in THP-1 cells. NaB and sodium propionate (NaPc), but not sodium acetate (NaAc), dramatically suppressed IL-1β and IFN-γ expression. The results indicated that NaB and NaPc could significantly inhibit the inflammation of THP-1 cells induced by A. baumannii, and the inhibitory effect was in the order of NaB > NaPc > NaAC. NaB and NaPc may inhibit inflammation through TLR-2/NF-κB/ROS/NLRP3 signaling pathway.

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来源期刊

Acta Pharmaceutica PHARMACOLOGY & PHARMACY-

CiteScore

5.20

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.