MitoTracker:一个需要更好替代品的有用工具

IF 4.5 3区生物学 Q2 CELL BIOLOGY

European journal of cell biology Pub Date : 2023-10-31 DOI:10.1016/j.ejcb.2023.151371

Kit Neikirk , Andrea G. Marshall , Bartosz Kula , Nathan Smith , Sharonda LeBlanc , Antentor Hinton Jr.

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引用次数: 1

摘要

线粒体的荧光观察通常由MitoTracker进行，这是一种由线粒体吸收的亲脂性阳离子染料。在这个论坛中，我们强调了MitoTracker可能出现的几个问题，包括其他细胞器的染色。我们的目的是提供替代染料，并讨论它们的优点和缺点。我们还提供替代MitoTracker的软件选项，以加快未来的实验设计。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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MitoTracker: A useful tool in need of better alternatives

The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.

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来源期刊

European journal of cell biology 生物-细胞生物学

CiteScore

7.30

自引率

1.50%

发文量

审稿时长

38 days

期刊介绍： The European Journal of Cell Biology, a journal of experimental cell investigation, publishes reviews, original articles and short communications on the structure, function and macromolecular organization of cells and cell components. Contributions focusing on cellular dynamics, motility and differentiation, particularly if related to cellular biochemistry, molecular biology, immunology, neurobiology, and developmental biology are encouraged. Manuscripts describing significant technical advances are also welcome. In addition, papers dealing with biomedical issues of general interest to cell biologists will be published. Contributions addressing cell biological problems in prokaryotes and plants are also welcome.