Flaminia Vincenti , Flavia Pagano , Camilla Montesano , Weronica K. Cipriani , Laura Micheli , Adolfo Gregori , Fabiana Di Rosa , Rosario Casamassima , Roberta Curini , Manuel Sergi
{"title":"hplc - hrms法测定法医样品中辣椒素类化合物的含量","authors":"Flaminia Vincenti , Flavia Pagano , Camilla Montesano , Weronica K. Cipriani , Laura Micheli , Adolfo Gregori , Fabiana Di Rosa , Rosario Casamassima , Roberta Curini , Manuel Sergi","doi":"10.1016/j.forc.2023.100527","DOIUrl":null,"url":null,"abstract":"<div><p>The use of Oleoresin Capsicum (OC)-based sprays has been reported as non-lethal self-defense tools in Europe since the 1950 s. Stinging pepper sprays may be very useful tool for self-defense in case of aggression, but they can turn into instruments of panic production with fatal outcomes, as demonstrated by what happened in the deadly Turin soccer stampede in 2017 or in Corinaldo (Ancona, Italy) nightclub in 2018.</p><p>The aim of the present work was the development and validation of an analytical method for the determination of the two most common capsaicinoids contained in OC-sprays at crime scenes. A simple and effective method for the extraction of capsaicinoids from commercial swabs, has been developed. Sample clean-up was carried out by μ-SPE, in order to reduce matrix effect. Qualitative and quantitative analysis was carried out by means of UHPLC-HRMS/MS.</p><p>The validated method was applied to the investigations related to the Corinaldo nightclub case, where a OC-spray was suspected of having created panic among the crowd, causing six dead and dozens hurt. A total number of 118 samples were collected from disco surfaces, including floors and balustrades, using swabs. The samples were treated and analysed with a validated method in order to identify and quantify the presence of capsaicinoids. Thanks to the large number of samples, it was possible to draw a concentration map of the analytes of interest within the disco, making possible to identify the room area from which the spray delivery started.</p></div>","PeriodicalId":324,"journal":{"name":"Forensic Chemistry","volume":"36 ","pages":"Article 100527"},"PeriodicalIF":2.6000,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination and quantification of capsaicin-type compounds in forensic samples by UHPLC-HRMS: The case of Corinaldo\",\"authors\":\"Flaminia Vincenti , Flavia Pagano , Camilla Montesano , Weronica K. Cipriani , Laura Micheli , Adolfo Gregori , Fabiana Di Rosa , Rosario Casamassima , Roberta Curini , Manuel Sergi\",\"doi\":\"10.1016/j.forc.2023.100527\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The use of Oleoresin Capsicum (OC)-based sprays has been reported as non-lethal self-defense tools in Europe since the 1950 s. Stinging pepper sprays may be very useful tool for self-defense in case of aggression, but they can turn into instruments of panic production with fatal outcomes, as demonstrated by what happened in the deadly Turin soccer stampede in 2017 or in Corinaldo (Ancona, Italy) nightclub in 2018.</p><p>The aim of the present work was the development and validation of an analytical method for the determination of the two most common capsaicinoids contained in OC-sprays at crime scenes. A simple and effective method for the extraction of capsaicinoids from commercial swabs, has been developed. Sample clean-up was carried out by μ-SPE, in order to reduce matrix effect. Qualitative and quantitative analysis was carried out by means of UHPLC-HRMS/MS.</p><p>The validated method was applied to the investigations related to the Corinaldo nightclub case, where a OC-spray was suspected of having created panic among the crowd, causing six dead and dozens hurt. A total number of 118 samples were collected from disco surfaces, including floors and balustrades, using swabs. The samples were treated and analysed with a validated method in order to identify and quantify the presence of capsaicinoids. Thanks to the large number of samples, it was possible to draw a concentration map of the analytes of interest within the disco, making possible to identify the room area from which the spray delivery started.</p></div>\",\"PeriodicalId\":324,\"journal\":{\"name\":\"Forensic Chemistry\",\"volume\":\"36 \",\"pages\":\"Article 100527\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic Chemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2468170923000632\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Chemistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468170923000632","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Determination and quantification of capsaicin-type compounds in forensic samples by UHPLC-HRMS: The case of Corinaldo
The use of Oleoresin Capsicum (OC)-based sprays has been reported as non-lethal self-defense tools in Europe since the 1950 s. Stinging pepper sprays may be very useful tool for self-defense in case of aggression, but they can turn into instruments of panic production with fatal outcomes, as demonstrated by what happened in the deadly Turin soccer stampede in 2017 or in Corinaldo (Ancona, Italy) nightclub in 2018.
The aim of the present work was the development and validation of an analytical method for the determination of the two most common capsaicinoids contained in OC-sprays at crime scenes. A simple and effective method for the extraction of capsaicinoids from commercial swabs, has been developed. Sample clean-up was carried out by μ-SPE, in order to reduce matrix effect. Qualitative and quantitative analysis was carried out by means of UHPLC-HRMS/MS.
The validated method was applied to the investigations related to the Corinaldo nightclub case, where a OC-spray was suspected of having created panic among the crowd, causing six dead and dozens hurt. A total number of 118 samples were collected from disco surfaces, including floors and balustrades, using swabs. The samples were treated and analysed with a validated method in order to identify and quantify the presence of capsaicinoids. Thanks to the large number of samples, it was possible to draw a concentration map of the analytes of interest within the disco, making possible to identify the room area from which the spray delivery started.
期刊介绍：
Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.
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