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Q3 Biochemistry, Genetics and Molecular Biology

Current Protocols in Cell Biology Pub Date : 2019-12-18 DOI:10.1002/cpcb.75

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摘要

封面:见Ravindranathan等人(https://doi.org/10.1002/cpcb.97)。RNAscope/PNA-FISH联合应用可检测单细胞TERT表达和端粒伸长。我们在tsq1或媒介感染后7天检测细胞。通过荧光检测野生型端粒(绿色)和TSQ1(红色)信号，然后通过亮场显微镜检测RNAscope TERT DAB反应产物。端粒共定位的TSQ1点用箭头标记。代表性图像显示，比例尺表示10µM。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cover: In Ravindranathan et al. (https://doi.org/10.1002/cpcb.97). Combined RNAscope/PNA-FISH can be used to detect TERT expression and telomere elongation in single cells. We assayed cells 7 days post-TSQ1 or vector infection. Cells were imaged via fluorescence for wild-type telomere (green) and TSQ1 (red) signal, and then via bright-field microscopy for the RNAscope TERT DAB reaction product. TSQ1 spots co-localizing at telomeres are marked with arrows. Representative images are shown, and scale bars denote 10 µM.

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Current Protocols in Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level. Updated every three months in all formats, CPCB is constantly evolving to keep pace with the very latest discoveries and developments.