碳源同化试验:传统方法与微技术(API)在肠杆菌科研究中的比较

F. Gavini , D. Izard , H. Leclerc , M. Desmonceaux , J.P. Gayral , avec la collaboration technique d’ A. Bernigaud
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引用次数: 19

摘要

采用两种方法研究了145种肠杆菌科细菌对不同碳源的同化作用:一种是传统的辅助造影法,另一种是专门为这些测试开发的标准化微法(API试纸)。对119种底物进行了比较,并对出现差异的试验进行了补充研究。在119项测试中,有108项发现了相关性。在其余情况下,补充研究表明,底物浓度的差异可以解释一些差异。由此得出结论,原料药试纸可以用来进行这些同化研究,底物浓度是最重要的可变因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Carbon Sources Assimilation Tests: Comparison Between a Conventional Method and a Microtechnic (API), in Study of Enterobacteriaceae

Assimilation of different carbon sources by 145 bacteria belonging to the family Enterobacteriaceae was studied by two methods: a conventional auxanographic method and a standardized micromethod (API strips) especially developed for these tests. 119 substrates were compared and complementary studies were carried out for tests showing discrepancies. A correlation was found for 108 of the 119 tests. In the remaining cases, complementary studies demonstrated that differences in substrate concentration may explain some discrepancies. It was concluded that API strips may be used to perform these assimilation studies and that substrate concentrations is the most important variable factor.

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