刺果蛾(Linnaeus, 1763)线粒体全基因组序列(鳞翅目:夜蛾总科)

Kuppusamy Sivasankaran , Pratheesh Mathew , Sekar Anand , Stanislaus Antony Ceasar , Soosaimanikam Mariapackiam , Savarimuthu Ignacimuthu
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引用次数: 11

摘要

对刺果蛾Eudocima phalonia (Linnaeus, 1763)(鳞翅目:夜蛾总科)线粒体全基因组进行了测序和鉴定(Genbank登录号:KY196412)。完整的有丝分裂基因组是一个长度为15,575 bp的圆形分子,由13个蛋白质编码基因(PCGs)、2个核糖体RNA基因(rrnS和rrnL)、22个转移RNA (tRNA)基因和一个a + t富区(D-loop)组成。基因组的核苷酸组成具有高度的A + T偏倚,占核苷酸的80.67%。所有tRNA都具有线粒体tRNA特有的二级结构。大多数PCGs是由典型的ATN密码子启动的。五个基因是由不寻常的密码子启动的。Cox1基因由一个不寻常的CGA密码子启动,由一个典型的终止密码子GAA终止。6个基因以单个t结尾,336 bp的a + t丰富区域由重复序列组成,包括2个ATAGA基序、1个19 bp的poly-T延伸和3个微卫星样区域((TA)4、(TA)6和2个(TA)7)。此外,在A + t富集区发现了3个大串联重复元件(1个40 bp和2个25 bp)。利用PCGs进行系统发育分析,发现超家族Noctuoidea是一个单系类群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complete mitochondrial genome sequence of fruit-piercing moth Eudocima phalonia (Linnaeus, 1763) (Lepidoptera: Noctuoidea)

The complete mitochondrial genome of the fruit piercing moth Eudocima phalonia (Linnaeus, 1763) (Lepidoptera: Noctuoidea) was sequenced and characterized (Genbank Accession No: KY196412). The complete mitogenome is a circular molecule of 15,575 bp length, consisting of 13 protein-coding genes (PCGs), two ribosomal RNA genes (rrnS and rrnL), 22 transfer RNA (tRNA) genes and an A + T-rich region (D-loop). The nucleotide composition of the genome is highly A + T biased, accounting for 80.67% of nucleotides. All tRNAs have putative secondary structures that are characteristic of mitochondrial tRNA. Most of the PCGs were initiated by typical ATN codons. Five genes were initiated by unusual codons. Cox1 gene was initiated by an unusual CGA codon and terminated by the typical stop codon GAA. Six genes ended with a single T. The A + T-rich region of 336 bp consisted of repetitive sequences, including two ATAGA motifs, a 19 bp poly-T stretch and three microsatellite-like regions ((TA)4, (TA)6 and two (TA)7). Moreover, three large tandem (one 40 bp and two 25 bp) repeated elements were identified in A + T-rich region. Phylogenetic analysis using PCGs revealed that Superfamily Noctuoidea is a monophyletic group.

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