John B Sutherland, James P Freeman, Anna J Williams, Carl E Cerniglia
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引用次数: 25
摘要
萨瑟兰，J. B.，弗里曼，J. P.，威廉姆斯，A. J.和Cerniglia, C. E. 1994。线虫对喹啉和异喹啉的n -氧化作用。实验真菌学18:271-274。线虫在含1.9mM喹啉或异喹啉的Sabouraud液体培养基中培养7 d。用乙酸乙酯提取废培养基;代谢产物采用高效液相色谱和薄层色谱分离纯化。通过HPLC洗脱时间、紫外/可见吸收光谱和质谱对各化合物的主要代谢物进行鉴定。在相似的条件下，大约65%添加的喹啉和3%添加的异喹啉分别代谢为喹啉N-氧化物和异喹啉N-氧化物。
N-Oxidation of Quinoline and Isoquinoline by Cunninghamella elegans
Sutherland, J. B., Freeman, J. P., Williams, A. J., and Cerniglia, C. E. 1994. N-oxidation of quinoline and isoquinoline by Cunninghamella elegans. Experimental Mycology 18: 271-274. Cultures of Cunninghamella elegans were grown for 7 days in liquid Sabouraud medium containing 1.9mM quinoline or isoquinoline. The spent culture media were extracted with ethyl acetate; metabolites were purified by high-performance liquid chromatography (HPLC) and thin-layer chromatography. The major metabolite produced from each compound was identified by the HPLC elution time, ultraviolet/visible absorption spectrum, and mass spectrum. Under similar conditions, approximately 65% of the added quinoline and 3% of the added isoquinoline were metabolized to quinoline N-oxide and isoquinoline N -oxide, respectively.
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