M. V. Efimova, Марина В. Ефимова, E. A. Mukhamatdinova, Е А Мухаматдинова, I. S. Kovtun, И С Ковтун, F. Kabil, Ф Кабил, Y. Medvedeva, Ю В Медведева, V. Kuznetsov, Виктор Валентинович Кузнецов
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引用次数: 1
摘要
在胁迫(100 mM NaCl)条件下评价茉莉酸(jasmonic acid, JA)的保护作用。以中季品种“卢格夫斯科伊”(Lugovskoy)的马铃薯植株为研究对象。将植株再生体嫁接到改良的Murashige-Skoog琼脂培养基中,在没有(对照)或存在JA(浓度为0.001)的情况下进行试管培养;在最优生长条件下或添加NaCl时,分别为0.1和10 M。培养28 d后,测定植株的生长(茎、根长、层数、叶数、植株质量)和生理(脯氨酸含量、光合色素含量、细胞渗出液渗透电位测定)。首次研究了茉莉酸(0.1和10 M)对盐胁迫下马铃薯植株的保护作用。盐渍化过程中,盐的部分去除对主要光合色素的负面影响和维持细胞内容物的渗透状态是保护作用的基础。
Jasmonic acid enhances the resistance of potato plants in vitro to salt stress
The protective effect of jasmonic acid (JA) was evaluated under stress (100 mM NaCl) condition. The investigations were carried on potato plants (Solanum tuberosum L.) of the mid-season variety Lugovskoy. Plant-regenerants were grafted and cultured in test tubes on modified Murashige-Skoog agar medium in the absence (control) or in the presence of JA at concentrations of 0.001; 0.1 and 10 M under optimal growing conditions or with the addition of NaCl. After 28 days of cultivation, growth (length of stem and root, number of tiers and leaves, plant mass) and physiological (proline content and photosynthetic pigments, determination of the osmotic potential of cell exudate) of the plants were assessed. For the first time it has been shown that jasmonic acid (0.1 and 10 M) manifests a pronounced protective effect on potato plants under salt stress condition. The protective effect based on the partial removal of the salt negative effect on the main photosynthetic pigments and the maintenance of the osmotic status of cell contents during salinization.