{"title":"光生物调节对肺部免疫因子mRNA表达水平的可能保护作用:一项扩展的COVID-19临床前和临床荟萃分析","authors":"Babak Arjmand, Fakher Rahim","doi":"10.1177/2632010X221127683","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Different expression of cytokine genes in the body determines the type of immune response (Th1 or Th2), which can play an important role in the pathogenesis of the COVID-19 disease.</p><p><strong>Aims: </strong>This meta-analysis was conducted to evaluate the probable effect of photobiomodulation (PBMT) therapy on the cytokine's mRNA expression in the lung.</p><p><strong>Methods: </strong>We systematically searched indexing databases, including PubMed/Medline, ISI web of science, Scopus, EMBASE, and Cochrane central, using standard terms without language, study region or type restrictions. Studies on using PBM in lung injury modeling with samples collected from lung tissue to observe IL-1β, TNF-α, IL-10, and IL-6 mRNA expression were included. RevMan 5.3 software was used for data analysis and standardized mean difference as effect size.</p><p><strong>Results: </strong>Of the 438 studies found through initial searches, 17 met the inclusion criteria. The main properties of 13 articles on 384 animals included in this meta-analysis with a wide range of species include rats (n = 10) and rabbits (n = 3). The analysis revealed that PBMT reduced the mRNA expression <i>of TNFα</i> (SMD: -3.70, 95% CI: -6.29, -1.11, <i>P</i> = .005,<i>I</i> <sup>2</sup> = 71%) and <i>IL-1β</i> (SMD: -5.85, 95% CI: -8.01, -3.69, <i>P</i> < .00001,<i>I</i> <sup>2</sup> = 37%) significantly, but no statistically significant reduction in <i>IL-6</i> (SMD: -2.89, 95% CI: -5.79, 0.01, <i>P</i> = .05,<i>I</i> <sup>2</sup> = 88%) was observed compared with the model controls. Also, PBMT increased <i>IL-10</i> mRNA expression significantly compared with the model controls (SMD: 1.04, 95% CI: 0.43, 1.64, <i>P</i> = .0008,<i>I</i> <sup>2</sup> = 17%).</p><p><strong>Conclusion: </strong>This meta-analysis revealed that the PBMT utilizes beneficial anti-inflammatory effects and modulation of the immune system on lung damage in animal models and clinical studies. 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Studies on using PBM in lung injury modeling with samples collected from lung tissue to observe IL-1β, TNF-α, IL-10, and IL-6 mRNA expression were included. RevMan 5.3 software was used for data analysis and standardized mean difference as effect size.</p><p><strong>Results: </strong>Of the 438 studies found through initial searches, 17 met the inclusion criteria. The main properties of 13 articles on 384 animals included in this meta-analysis with a wide range of species include rats (n = 10) and rabbits (n = 3). The analysis revealed that PBMT reduced the mRNA expression <i>of TNFα</i> (SMD: -3.70, 95% CI: -6.29, -1.11, <i>P</i> = .005,<i>I</i> <sup>2</sup> = 71%) and <i>IL-1β</i> (SMD: -5.85, 95% CI: -8.01, -3.69, <i>P</i> < .00001,<i>I</i> <sup>2</sup> = 37%) significantly, but no statistically significant reduction in <i>IL-6</i> (SMD: -2.89, 95% CI: -5.79, 0.01, <i>P</i> = .05,<i>I</i> <sup>2</sup> = 88%) was observed compared with the model controls. 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引用次数: 0
摘要
背景:机体细胞因子基因的不同表达决定了免疫反应的类型(Th1或Th2)，在COVID-19疾病的发病机制中发挥重要作用。目的:本荟萃分析旨在评估光生物调节(PBMT)治疗对肺细胞因子mRNA表达的可能影响。方法:我们系统地检索索引数据库，包括PubMed/Medline、ISI web of science、Scopus、EMBASE和Cochrane central，使用标准术语，没有语言、研究区域或类型限制。采用PBM建立肺损伤模型，观察肺组织中IL-1β、TNF-α、IL-10和IL-6 mRNA的表达。采用RevMan 5.3软件进行数据分析，采用标准化均差作为效应量。结果:在初步检索的438项研究中，有17项符合纳入标准。本荟萃分析纳入了13篇文章，涉及384种动物，主要特性包括大鼠(n = 10)和兔子(n = 3)。分析显示，PBMT降低了tnf - α mRNA的表达(SMD: -3.70, 95% CI: -6.29， -1.11, P =。005, i2 = 71%)和IL-1β (SMD: -5.85, 95% CI: -8.01， -3.69, ip2 = 37%)显著降低，但IL-6 (SMD: -2.89, 95% CI: -5.79, 0.01, P =。0.05, i2 = 88%)。与模型对照组相比，PBMT显著增加了IL-10 mRNA的表达(SMD: 1.04, 95% CI: 0.43, 1.64, P =。0008, i 2 = 17%)。结论:本荟萃分析显示，在动物模型和临床研究中，PBMT具有有益的抗炎作用和免疫系统调节肺损伤。然而，考虑到证据的质量，动物模型和临床研究似乎有限;因此，仍需要大规模的临床试验。
The Probable Protective Effect of Photobiomodulation on the Immunologic Factor's mRNA Expression Level in the Lung: An Extended COVID-19 Preclinical and Clinical Meta-analysis.
Background: Different expression of cytokine genes in the body determines the type of immune response (Th1 or Th2), which can play an important role in the pathogenesis of the COVID-19 disease.
Aims: This meta-analysis was conducted to evaluate the probable effect of photobiomodulation (PBMT) therapy on the cytokine's mRNA expression in the lung.
Methods: We systematically searched indexing databases, including PubMed/Medline, ISI web of science, Scopus, EMBASE, and Cochrane central, using standard terms without language, study region or type restrictions. Studies on using PBM in lung injury modeling with samples collected from lung tissue to observe IL-1β, TNF-α, IL-10, and IL-6 mRNA expression were included. RevMan 5.3 software was used for data analysis and standardized mean difference as effect size.
Results: Of the 438 studies found through initial searches, 17 met the inclusion criteria. The main properties of 13 articles on 384 animals included in this meta-analysis with a wide range of species include rats (n = 10) and rabbits (n = 3). The analysis revealed that PBMT reduced the mRNA expression of TNFα (SMD: -3.70, 95% CI: -6.29, -1.11, P = .005,I2 = 71%) and IL-1β (SMD: -5.85, 95% CI: -8.01, -3.69, P < .00001,I2 = 37%) significantly, but no statistically significant reduction in IL-6 (SMD: -2.89, 95% CI: -5.79, 0.01, P = .05,I2 = 88%) was observed compared with the model controls. Also, PBMT increased IL-10 mRNA expression significantly compared with the model controls (SMD: 1.04, 95% CI: 0.43, 1.64, P = .0008,I2 = 17%).
Conclusion: This meta-analysis revealed that the PBMT utilizes beneficial anti-inflammatory effects and modulation of the immune system on lung damage in animal models and clinical studies. However, animal models and clinical studies appear limited considering the evidence's quality; therefore, large clinical trials are still required.
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