海洋酸化是否会影响褐海螺的消化生理和肠道微生物群?

IF 0.5 4区 农林科学 Q4 FISHERIES
Fen Cheng, H. Vasquez, Shouguo Yang, Xiangyu Wu, Y. Xing, Xianming Tang
{"title":"海洋酸化是否会影响褐海螺的消化生理和肠道微生物群?","authors":"Fen Cheng, H. Vasquez, Shouguo Yang, Xiangyu Wu, Y. Xing, Xianming Tang","doi":"10.46989/001c.72706","DOIUrl":null,"url":null,"abstract":"To understand the physiological responses of the Brunneifusus ternatanus to future ocean acidification (OA), histology, enzyme activity and gut bacterial composition at different pH levels (Control: C group, pH 8.1; Exposure period: EP group, pH 7.3) for 28 days were studied under laboratory conditions. Microbiota composition was analyzed using 16S rRNA gene amplicon sequencing. Enzyme activities of trypsin (TRY), lipase (LPS), amylase (AMS), and lysozyme (LZM) were used as biochemical indicators, as well as weight gain rate (WGR), specific growth rate (SGR) as growth indicators. The stress caused by OA resulted in alterations to the intestine, including partially swollen and degranulated enterocytes and rough endoplasmic reticulum (RER). The relative abundance of the core phylum in the acidified group changed significantly, showing an increase in Tenericutes and a decrease in Proteobacteria. Firmicutes/Bacteroides ratio declined from 4.38 in the control group to 1.25 in the EP group. We found that the enzymes TRY, LPS, and AMS activities were inhibited at reduced pH, which was positively correlated with the dominant genera Mycoplasma and Bacteroides; while LZM activities showed a significant increment, but showing a strong negative correlation. Furthermore, both WG and SRG values showed a depression at low pH lever. These results suggest that if anthropogenic CO2 emissions continue to accelerate, OA could negatively impact the whelk’s health, compromising their growth performance and even survival. These findings will benefit the future risk assessments of OA or other related emerging environmental issues.","PeriodicalId":14704,"journal":{"name":"Israeli Journal of Aquaculture-bamidgeh","volume":"70 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Will ocean acidification affect the digestive physiology and gut microbiota of whelk Brunneifusus ternatanus?\",\"authors\":\"Fen Cheng, H. Vasquez, Shouguo Yang, Xiangyu Wu, Y. Xing, Xianming Tang\",\"doi\":\"10.46989/001c.72706\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To understand the physiological responses of the Brunneifusus ternatanus to future ocean acidification (OA), histology, enzyme activity and gut bacterial composition at different pH levels (Control: C group, pH 8.1; Exposure period: EP group, pH 7.3) for 28 days were studied under laboratory conditions. Microbiota composition was analyzed using 16S rRNA gene amplicon sequencing. Enzyme activities of trypsin (TRY), lipase (LPS), amylase (AMS), and lysozyme (LZM) were used as biochemical indicators, as well as weight gain rate (WGR), specific growth rate (SGR) as growth indicators. The stress caused by OA resulted in alterations to the intestine, including partially swollen and degranulated enterocytes and rough endoplasmic reticulum (RER). The relative abundance of the core phylum in the acidified group changed significantly, showing an increase in Tenericutes and a decrease in Proteobacteria. Firmicutes/Bacteroides ratio declined from 4.38 in the control group to 1.25 in the EP group. We found that the enzymes TRY, LPS, and AMS activities were inhibited at reduced pH, which was positively correlated with the dominant genera Mycoplasma and Bacteroides; while LZM activities showed a significant increment, but showing a strong negative correlation. Furthermore, both WG and SRG values showed a depression at low pH lever. These results suggest that if anthropogenic CO2 emissions continue to accelerate, OA could negatively impact the whelk’s health, compromising their growth performance and even survival. These findings will benefit the future risk assessments of OA or other related emerging environmental issues.\",\"PeriodicalId\":14704,\"journal\":{\"name\":\"Israeli Journal of Aquaculture-bamidgeh\",\"volume\":\"70 1\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2023-03-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Israeli Journal of Aquaculture-bamidgeh\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.46989/001c.72706\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Israeli Journal of Aquaculture-bamidgeh","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.46989/001c.72706","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

摘要

为了解不同pH水平下ternatanus Brunneifusus对未来海洋酸化(OA)的生理反应、组织学、酶活性和肠道细菌组成(Control: C组,pH 8.1;暴露期:实验室条件下EP组(pH 7.3) 28 d。采用16S rRNA基因扩增子测序分析菌群组成。以胰蛋白酶(TRY)、脂肪酶(LPS)、淀粉酶(AMS)、溶菌酶(LZM)活性为生化指标,以增重率(WGR)、特定生长率(SGR)为生长指标。OA引起的应激导致肠道的改变,包括部分肿胀和脱颗粒的肠细胞和粗糙的内质网(RER)。酸化组核心门的相对丰度发生了显著变化,Tenericutes增加,Proteobacteria减少。厚壁菌门/拟杆菌门比值由对照组的4.38降至EP组的1.25。结果表明,在降低pH条件下,菌株的TRY、LPS和AMS活性均受到抑制,且与支原体和拟杆菌属的优势菌群呈正相关;LZM活动显著增加,但呈较强的负相关。此外,在低pH水平下,WG和SRG值均呈下降趋势。这些结果表明,如果人为的二氧化碳排放继续加速,OA可能会对海螺的健康产生负面影响,影响它们的生长性能甚至生存。这些研究结果将有助于未来对OA或其他相关新出现的环境问题进行风险评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Will ocean acidification affect the digestive physiology and gut microbiota of whelk Brunneifusus ternatanus?
To understand the physiological responses of the Brunneifusus ternatanus to future ocean acidification (OA), histology, enzyme activity and gut bacterial composition at different pH levels (Control: C group, pH 8.1; Exposure period: EP group, pH 7.3) for 28 days were studied under laboratory conditions. Microbiota composition was analyzed using 16S rRNA gene amplicon sequencing. Enzyme activities of trypsin (TRY), lipase (LPS), amylase (AMS), and lysozyme (LZM) were used as biochemical indicators, as well as weight gain rate (WGR), specific growth rate (SGR) as growth indicators. The stress caused by OA resulted in alterations to the intestine, including partially swollen and degranulated enterocytes and rough endoplasmic reticulum (RER). The relative abundance of the core phylum in the acidified group changed significantly, showing an increase in Tenericutes and a decrease in Proteobacteria. Firmicutes/Bacteroides ratio declined from 4.38 in the control group to 1.25 in the EP group. We found that the enzymes TRY, LPS, and AMS activities were inhibited at reduced pH, which was positively correlated with the dominant genera Mycoplasma and Bacteroides; while LZM activities showed a significant increment, but showing a strong negative correlation. Furthermore, both WG and SRG values showed a depression at low pH lever. These results suggest that if anthropogenic CO2 emissions continue to accelerate, OA could negatively impact the whelk’s health, compromising their growth performance and even survival. These findings will benefit the future risk assessments of OA or other related emerging environmental issues.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.90
自引率
16.70%
发文量
49
审稿时长
3 months
期刊介绍: Information not localized
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信