经皮免疫调节对肝脏再生的影响

E. G. Kuznetsova, O. M. Kuryleva, L. A. Salomatina, L. A. Kirsanova, Z. Z. Gonikova, A. Nikolskaya, N. Shmerko, V. Sevastianov
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摘要

介绍。使用免疫调节剂来调节受影响器官和组织的修复过程仍然是一个紧迫的问题。最令人感兴趣的是右叶活体肝移植供体延长肝切除(EHR)后的肝再生。我们提出一种含免疫调节剂的经皮治疗系统(TTS)来促进肝组织再生的自然过程。目的:通过体内实验研究免疫调节剂氨基二氢酞嗪二酮钠经皮给药对EHR术后肝脏早期恢复过程的影响。材料和方法。氨基二氢酞嗪二酮钠以粉末形式作为TTS中的活性物质,用于制备肌内注射溶液(Galavit®,SELVIM LLC)。取22只体重350 ~ 380 g的雄性Wistar大鼠建立实验性EHR模型。在HER之后,所有的动物被分成两组。第1组(n = 10)为未经治疗的动物。组2 (n = 12)在肝切除术后立即应用TTS。试验期48 h;TTS在给药24小时后改变一次。两组在EHR后48 h的残肝增重和血液生化指标均无显著差异。EHR后48小时肝细胞有丝分裂指数(MI)的评估显示,与基线(肝切除前)相比,两组的MI显著增加,等于0.14±0.07‰。1组和2组的心肌梗死发生率分别为12.70±4.9‰和17.43±4.90‰(p≤0.05)。对氨基二氢酞嗪二酮钠TTS在实验性EHR大鼠模型上的再生活性研究表明,该药物形式对肝细胞有丝分裂活性有明显的刺激作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of transdermal immunomodulation on liver regeneration
Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.
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