Kay Ann S. Jonatas, Joseph Mari B. Querequincia, Shiela Dv Miranda, U. Obatavwe, M. Corpuz, R. D. Vasquez
{"title":"香树果的抗糖尿病作用评价","authors":"Kay Ann S. Jonatas, Joseph Mari B. Querequincia, Shiela Dv Miranda, U. Obatavwe, M. Corpuz, R. D. Vasquez","doi":"10.20885/jif.vol16.iss1.art1","DOIUrl":null,"url":null,"abstract":"Background: Diabetes mellitus causes 4.2 million of deaths worldwide and 79% adults with diabetes are living in low- and middle-income countries. This research providing an alternative therapy through the prevention of postprandial hyperglycemia may help diabetic patients and provide a new utilization model of fruit peel. Artocarpus odoratissimus , commonly known as marang, is an edible fruit found in the southern part of the Philippines. Most of the weight of the fruit is discarded and treated as waste. Objectives: This study aimed to utilize the by-products of marang fruit as a promising pharmaceutical agent by determining the phytochemicals present and in vitro antidiabetic activity of the different parts of the fruit. Methods: Phytochemical screening of phenolics and flavonoids was done through thin layer chromatography. Ten concentrations (2-1000 µg/mL) of the extracts from the peel, pulp, and seeds were evaluated for the in vitro antidiabetic assay using alpha-glucosidase enzyme. Mean percent inhibition was calculated, and data was analyzed using ANOVA. The IC 50 estimates were calculated using the program GraphPad Prism version 8. Results: Extracts from the fruit parts of A. odoratissimus contained phenols and flavonoids and were active inhibitors of alpha-glucosidase enzyme. The fruit peel extract of marang was the most potent (IC 50 = 48.19 µg/mL) compared to the seed extract, pulp extract, and the standard drug acarbose (p value = 0.035). Conclusion: The fruit waste, the peel and seeds, has an intense activity against alpha-glucosidase enzyme because of their phenols and flavonoid contents. Keywords: alpha-glucosidase, Artocarpus, diabetes, phenolics, fruit peel","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Antidiabetic evaluation of Artocarpus odoratissimus (Moraceae) fruit\",\"authors\":\"Kay Ann S. Jonatas, Joseph Mari B. Querequincia, Shiela Dv Miranda, U. Obatavwe, M. Corpuz, R. D. Vasquez\",\"doi\":\"10.20885/jif.vol16.iss1.art1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Diabetes mellitus causes 4.2 million of deaths worldwide and 79% adults with diabetes are living in low- and middle-income countries. This research providing an alternative therapy through the prevention of postprandial hyperglycemia may help diabetic patients and provide a new utilization model of fruit peel. Artocarpus odoratissimus , commonly known as marang, is an edible fruit found in the southern part of the Philippines. Most of the weight of the fruit is discarded and treated as waste. Objectives: This study aimed to utilize the by-products of marang fruit as a promising pharmaceutical agent by determining the phytochemicals present and in vitro antidiabetic activity of the different parts of the fruit. Methods: Phytochemical screening of phenolics and flavonoids was done through thin layer chromatography. Ten concentrations (2-1000 µg/mL) of the extracts from the peel, pulp, and seeds were evaluated for the in vitro antidiabetic assay using alpha-glucosidase enzyme. Mean percent inhibition was calculated, and data was analyzed using ANOVA. The IC 50 estimates were calculated using the program GraphPad Prism version 8. Results: Extracts from the fruit parts of A. odoratissimus contained phenols and flavonoids and were active inhibitors of alpha-glucosidase enzyme. The fruit peel extract of marang was the most potent (IC 50 = 48.19 µg/mL) compared to the seed extract, pulp extract, and the standard drug acarbose (p value = 0.035). Conclusion: The fruit waste, the peel and seeds, has an intense activity against alpha-glucosidase enzyme because of their phenols and flavonoid contents. Keywords: alpha-glucosidase, Artocarpus, diabetes, phenolics, fruit peel\",\"PeriodicalId\":32369,\"journal\":{\"name\":\"Kartika Jurnal Ilmiah Farmasi\",\"volume\":\"59 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kartika Jurnal Ilmiah Farmasi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20885/jif.vol16.iss1.art1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kartika Jurnal Ilmiah Farmasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20885/jif.vol16.iss1.art1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antidiabetic evaluation of Artocarpus odoratissimus (Moraceae) fruit
Background: Diabetes mellitus causes 4.2 million of deaths worldwide and 79% adults with diabetes are living in low- and middle-income countries. This research providing an alternative therapy through the prevention of postprandial hyperglycemia may help diabetic patients and provide a new utilization model of fruit peel. Artocarpus odoratissimus , commonly known as marang, is an edible fruit found in the southern part of the Philippines. Most of the weight of the fruit is discarded and treated as waste. Objectives: This study aimed to utilize the by-products of marang fruit as a promising pharmaceutical agent by determining the phytochemicals present and in vitro antidiabetic activity of the different parts of the fruit. Methods: Phytochemical screening of phenolics and flavonoids was done through thin layer chromatography. Ten concentrations (2-1000 µg/mL) of the extracts from the peel, pulp, and seeds were evaluated for the in vitro antidiabetic assay using alpha-glucosidase enzyme. Mean percent inhibition was calculated, and data was analyzed using ANOVA. The IC 50 estimates were calculated using the program GraphPad Prism version 8. Results: Extracts from the fruit parts of A. odoratissimus contained phenols and flavonoids and were active inhibitors of alpha-glucosidase enzyme. The fruit peel extract of marang was the most potent (IC 50 = 48.19 µg/mL) compared to the seed extract, pulp extract, and the standard drug acarbose (p value = 0.035). Conclusion: The fruit waste, the peel and seeds, has an intense activity against alpha-glucosidase enzyme because of their phenols and flavonoid contents. Keywords: alpha-glucosidase, Artocarpus, diabetes, phenolics, fruit peel