{"title":"LC-MS/MS法测定生物基质中盐酸依拉瓦环素的含量","authors":"B. S. Prasad, Sekharan Jaya Kumari","doi":"10.5530/PHM.2019.2.9","DOIUrl":null,"url":null,"abstract":"Objective: The validated protein precipitation method was applied for estimation of Eravacycline dihydrochloride in human plasma with Rolitetracycline hydrochloride as an internal standard (ISTD) by using HPLC-ESI-MS/ MS. Methods: The chromatographic separation was achieved with 20mM Ammonium acetate (pH-3.0):Methanol:Acetontrile (20:20:60,%v/v) using the TELOS LU C18 (2) 5μm, 100 x 4.6 mm. The total analysis time was 3 min and flow rate was set to 0.5 mL/min. Results: The mass transitions of Eravacycline dihydrochloride and Rolitetracycline hydrochloride obtained were m/z 632.5®84.3 and 436.2®84.3. The standard curve shows correlation coefficient (s) greater than 0.999 with a range of 15.00-120.00 pg/ml using the linear regression model. Conclusion: The method was suitable and conveniently applicable to pharmacokinetic and bioavailability studies for estimation of Eravacycline in biological matrices by HPLC-ESI-MS/MS.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"26 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Estimation of Eravacycline Dihydrochloride in Biological Matrices by LC-MS/MS\",\"authors\":\"B. S. Prasad, Sekharan Jaya Kumari\",\"doi\":\"10.5530/PHM.2019.2.9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: The validated protein precipitation method was applied for estimation of Eravacycline dihydrochloride in human plasma with Rolitetracycline hydrochloride as an internal standard (ISTD) by using HPLC-ESI-MS/ MS. Methods: The chromatographic separation was achieved with 20mM Ammonium acetate (pH-3.0):Methanol:Acetontrile (20:20:60,%v/v) using the TELOS LU C18 (2) 5μm, 100 x 4.6 mm. The total analysis time was 3 min and flow rate was set to 0.5 mL/min. Results: The mass transitions of Eravacycline dihydrochloride and Rolitetracycline hydrochloride obtained were m/z 632.5®84.3 and 436.2®84.3. The standard curve shows correlation coefficient (s) greater than 0.999 with a range of 15.00-120.00 pg/ml using the linear regression model. Conclusion: The method was suitable and conveniently applicable to pharmacokinetic and bioavailability studies for estimation of Eravacycline in biological matrices by HPLC-ESI-MS/MS.\",\"PeriodicalId\":19960,\"journal\":{\"name\":\"Pharmaceutical Methods\",\"volume\":\"26 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceutical Methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5530/PHM.2019.2.9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical Methods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/PHM.2019.2.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
目的:以盐酸罗利特环素为内标(ISTD)，采用高效液相色谱- esi -质谱联用技术(HPLC-ESI-MS/ ms)测定人血浆中盐酸依拉瓦环素的含量。方法:采用20mM乙酸铵(pH-3.0):甲醇:乙腈(20:20:60，%v/v)，色谱柱为TELOS LU C18 (2) 5μm, 100 × 4.6 mm。总分析时间为3 min，流速为0.5 mL/min。结果:得到的盐酸依拉瓦环素和盐酸罗利特环素的质量转移率分别为m/z 632.5®84.3和436.2®84.3。在15.00 ~ 120.00 pg/ml的线性回归范围内，标准曲线的相关系数大于0.999。结论:该方法适用于高效液相色谱- esi -MS/MS法测定伊拉瓦环素在生物基质中的药动学和生物利用度研究。
Estimation of Eravacycline Dihydrochloride in Biological Matrices by LC-MS/MS
Objective: The validated protein precipitation method was applied for estimation of Eravacycline dihydrochloride in human plasma with Rolitetracycline hydrochloride as an internal standard (ISTD) by using HPLC-ESI-MS/ MS. Methods: The chromatographic separation was achieved with 20mM Ammonium acetate (pH-3.0):Methanol:Acetontrile (20:20:60,%v/v) using the TELOS LU C18 (2) 5μm, 100 x 4.6 mm. The total analysis time was 3 min and flow rate was set to 0.5 mL/min. Results: The mass transitions of Eravacycline dihydrochloride and Rolitetracycline hydrochloride obtained were m/z 632.5®84.3 and 436.2®84.3. The standard curve shows correlation coefficient (s) greater than 0.999 with a range of 15.00-120.00 pg/ml using the linear regression model. Conclusion: The method was suitable and conveniently applicable to pharmacokinetic and bioavailability studies for estimation of Eravacycline in biological matrices by HPLC-ESI-MS/MS.
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