种子植物特有的SNARE复合物对拟南芥蛋白质储存液泡生物发生和种子发育的影响[W][OA]

Kazuo Ebine, Yusuke Okatani, T. Uemura, T. Goh, Keiko Shoda, Mitsuru Niihama, M. Morita, Christoph Spitzer, M. Otegui, A. Nakano, T. Ueda
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引用次数: 192

摘要

SNARE复合体是囊泡运输的关键调节器,执行运输囊泡或细胞器与靶膜之间的膜融合。一个功能性的SNARE复合体由四个螺旋束组成,其中三个由q -SNARE提供,另一个由R-SNARE提供。拟南芥VAMP727是一个R-SNARE,仅在种子植物中有同源物。我们发现VAMP727与SYP22/ VAM3(一个Q-SNARE)共定位于与液泡膜密切相关的泡前室/核内体亚群上。遗传和生化分析,包括检查vamp727和syp22突变的协同相互作用,蛋白质定位的组织学检查以及来自拟南芥分离物的共免疫沉淀表明,vamp727与syp22, VTI11和SYP51形成复合物,该复合物在液泡运输,种子成熟和液泡生物发生中起着至关重要的作用。我们认为VAMP727复合物介导了泡前室和液泡之间的膜融合,这一过程已经进化为种子发育的重要步骤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana[W][OA]
The SNARE complex is a key regulator of vesicular traffic, executing membrane fusion between transport vesicles or organelles and target membranes. A functional SNARE complex consists of four coiled-coil helical bundles, three of which are supplied by Q-SNAREs and another from an R-SNARE. Arabidopsis thaliana VAMP727 is an R-SNARE, with homologs only in seed plants. We have found that VAMP727 colocalizes with SYP22/ VAM3, a Q-SNARE, on a subpopulation of prevacuolar compartments/endosomes closely associated with the vacuolar membrane. Genetic and biochemical analyses, including examination of a synergistic interaction of vamp727 and syp22 mutations, histological examination of protein localization, and coimmunoprecipitation from Arabidopsis lysates indicate that VAMP727 forms a complex with SYP22, VTI11, and SYP51 and that this complex plays a crucial role in vacuolar transport, seed maturation, and vacuole biogenesis. We suggest that the VAMP727 complex mediates the membrane fusion between the prevacuolar compartment and the vacuole and that this process has evolved as an essential step for seed development.
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