{"title":"在卡杜纳售卖及食用的虎果中发现黄曲霉毒素","authors":"A. Shukla, M. Mehrotra","doi":"10.18535/IJSRE/V4I04.13","DOIUrl":null,"url":null,"abstract":"Twenty (20) samples of tiger nut collected from different food stuff markets in Kaduna were subjected to mycological analysis to determine the fungal load and to isolate the aflatoxin producing organisms and to analyze for aflatoxin presence using thin layer chromatography (TLC), enzyme linked immunosorbent assay (ELISA), and fourier transform infrared spectroscopy (FTIR) techniques. The result of the mycological analysis showed that the samples had an average fungal load of 1.8 X 10 3 cfug -1 and the aflatoxin producing fungi isolated were Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. The TLC result showed that two of the samples analysed yielded blue fluorescence spots on the plate. The ELISA result showed that seven samples contained aflatoxin with concentration varying from 0.2µg/kg to 23.0µg/kg. The result is then confirmed by the detection of the functional groups , C=O, C-O, and C-H which are found in the structure of aflatoxin using FTIR.","PeriodicalId":14282,"journal":{"name":"International Journal of Scientific Research in Education","volume":"54 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Occurrence of Aflatoxin in Cyperus esculentus (Tiger Nut) Sold and Consumed Raw in Kaduna\",\"authors\":\"A. Shukla, M. Mehrotra\",\"doi\":\"10.18535/IJSRE/V4I04.13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Twenty (20) samples of tiger nut collected from different food stuff markets in Kaduna were subjected to mycological analysis to determine the fungal load and to isolate the aflatoxin producing organisms and to analyze for aflatoxin presence using thin layer chromatography (TLC), enzyme linked immunosorbent assay (ELISA), and fourier transform infrared spectroscopy (FTIR) techniques. The result of the mycological analysis showed that the samples had an average fungal load of 1.8 X 10 3 cfug -1 and the aflatoxin producing fungi isolated were Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. The TLC result showed that two of the samples analysed yielded blue fluorescence spots on the plate. The ELISA result showed that seven samples contained aflatoxin with concentration varying from 0.2µg/kg to 23.0µg/kg. The result is then confirmed by the detection of the functional groups , C=O, C-O, and C-H which are found in the structure of aflatoxin using FTIR.\",\"PeriodicalId\":14282,\"journal\":{\"name\":\"International Journal of Scientific Research in Education\",\"volume\":\"54 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-04-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Scientific Research in Education\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18535/IJSRE/V4I04.13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Scientific Research in Education","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18535/IJSRE/V4I04.13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
采用薄层色谱(TLC)、酶联免疫吸附法(ELISA)和傅里叶变换红外光谱(FTIR)技术对从卡杜纳不同食品市场采集的20份虎坚果样品进行真菌学分析，以确定真菌负荷，分离产生黄曲霉毒素的微生物，并分析黄曲霉毒素的存在。真菌学分析结果表明，样品的平均真菌负荷为1.8 X 10 3 cfug -1，分离到的产黄曲霉主要为黄曲霉、寄生曲霉和绿曲霉。薄层色谱结果显示，分析的两个样品在板上产生蓝色荧光点。ELISA检测结果显示，7份样品中含有黄曲霉毒素，浓度范围为0.2µg/kg ~ 23.0µg/kg。然后用FTIR检测黄曲霉毒素结构中的官能团C=O、C-O和C- h，证实了这一结果。
Occurrence of Aflatoxin in Cyperus esculentus (Tiger Nut) Sold and Consumed Raw in Kaduna
Twenty (20) samples of tiger nut collected from different food stuff markets in Kaduna were subjected to mycological analysis to determine the fungal load and to isolate the aflatoxin producing organisms and to analyze for aflatoxin presence using thin layer chromatography (TLC), enzyme linked immunosorbent assay (ELISA), and fourier transform infrared spectroscopy (FTIR) techniques. The result of the mycological analysis showed that the samples had an average fungal load of 1.8 X 10 3 cfug -1 and the aflatoxin producing fungi isolated were Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius. The TLC result showed that two of the samples analysed yielded blue fluorescence spots on the plate. The ELISA result showed that seven samples contained aflatoxin with concentration varying from 0.2µg/kg to 23.0µg/kg. The result is then confirmed by the detection of the functional groups , C=O, C-O, and C-H which are found in the structure of aflatoxin using FTIR.
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