在雄性大鼠中,同时暴露于香烟烟雾和煤尘可诱导肺氧化应激并降低血清MUC5AC水平

Nia Kania , Bambang Setiawan , Edi Widjajanto , Nurdiana Nurdiana , M. Aris Widodo , H.M.S. Chandra Kusuma
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引用次数: 1

摘要

本研究旨在探讨同时暴露于香烟烟雾和煤尘是否会激活表皮生长因子受体(EGFR) MUC5AC的表达。32只雄性Wistar大鼠分为两组,每组8只:对照组(C);暴露于香烟烟雾加煤尘剂量为6.25 mg/m3 (CS + CD1);12.5 mg/m3 (CS + CD2);25 mg/m3 (CS + CD3)。暴露时间21天,1小时/天。用比色法分析肺丙二醛水平。ELISA法检测血清EGF和MUC5AC的表达。共聚焦激光扫描显微镜检测肺EGFR和MUC5AC的表达。与对照组相比,所有暴露剂量的肺丙二醛水平均显著升高(p <0.05)。与对照组和CS + CD1组相比,CS + CD2组血清EGF水平显著升高。各治疗组间EGFR表达差异无统计学意义(p >0.05)。与对照组相比,两个最高剂量煤尘组血清MUC5AC水平显著降低。综上所述,亚慢性联合暴露可引起肺氧化应激和炎症反应,降低血清MUC5AC水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Concomitant exposure to cigarette smoke and coal dust induces lung oxidative stress and decreases serum MUC5AC levels in male rats

This study aimed to investigate whether concomitant exposure to cigarette smoke and coal dust could activate the epidermal growth factor receptor (EGFR) for MUC5AC expression. Thirty-two male Wistar rats were divided into the following groups (n = 8 each): control group (C); exposed to cigarette smoke plus coal dust at doses of 6.25 mg/m3 (CS + CD1); 12.5 mg/m3 (CS + CD2); and 25 mg/m3 (CS + CD3). The duration of exposure was 21 days, 1 hour/day. Lung malondialdehyde level was analyzed colorimetrically. Serum EGF and MUC5AC expression were measured by ELISA. Expression of lung EGFR and MUC5AC were measured by a confocal laser scanning microscopy. The level of lung malondialdehyde was higher significantly in all doses of exposure compared with control group (p < 0.05). The level of serum EGF was significantly increased in CS + CD2 group compared with control group or CS + CD1 group. The expression of EGFR was not significantly different among all the treatment groups (p > 0.05). Serum MUC5AC levels were significantly lower in the two highest doses of coal dust compared with the control group. In conclusion, subchronic combined exposure to cigarette smoke and coal dust induces lung oxidative stress and inflammation and decreases serum MUC5AC level.

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