番茄斑病病毒在辣椒抗性和易感种质中的运动和分布。

S. Soler, M. Díez, S. Roselló, F. Nuez
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引用次数: 22

摘要

以抗性辣椒材料PI-152225、PI-159236和敏感辣椒品种“Negral”为材料,通过3个试验对辣椒材料对番茄斑枯病毒(TSWV)的抗性进行了表征。在第一个实验中,研究了2叶期和4叶期全株的病毒运动。在第二个试验中,分析了接种叶片在3个生育期(2叶、3叶和4叶期)内的运动。本实验采用双抗体夹心酶联免疫吸附法(DAS-ELISA)和直接组织印迹法(DTB)两种技术。后一实验采用DAS-ELISA法测定病毒在不同类型样品中的积累情况。DTB技术表明,病毒在接种叶片内的运动在抗性植株中受到限制。在抗性品种中,接种区未被完全感染,病毒在接种区内的运动速度较慢。疫苗接种中ELISA弱阳性的检测
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Movement and distribution of tomato spotted wilt virus in resistant and susceptible accessions of Capsicum spp.
Resistant Capsicum chinense accessions PI-152225 and PI-159236 and the susceptible cultivar ‘Negral’ of Capsicum annuum were used in three experiments to characterize the resistance to tomato spotted wilt virus (TSWV) in these materials. In the first experiment viral movement in the whole plant was studied at two growth stages (2- and 4-leaf stage). In the second experiment the movement within the inoculated leaf was analysed at three growth stages (2-, 3-, and 4-leaf stage). Two techniques were used in this assay: double antibody sandwich – enzyme-linked immunosorbent assay (DAS–ELISA) and direct tissue blotting (DTB). In the latter experiment viral accumulation in different types of samples was evaluated by DAS–ELISA. The DTB technique showed that viral movement within the inoculated leaf is restricted in the resistant plants. The inoculated area was not totally infected in resistant accessions and slower viral movement within the inoculated area was observed. Detection of weak ELISA positives in inocul...
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