E. Maslova, Tatyana Glodik, Valeria Semykina, Natalya Gulya, T. Perelygina
{"title":"草鼠尾草愈伤组织体外抗菌活性研究","authors":"E. Maslova, Tatyana Glodik, Valeria Semykina, Natalya Gulya, T. Perelygina","doi":"10.2991/isils-19.2019.49","DOIUrl":null,"url":null,"abstract":"—In this paper, we studied the antimicrobial activity of callus tissue Salvia pratensis L. obtained in vitro against Escherichia coli and Staphylococcus aureus . The most effective sterilizing agents for disinfection of plant explants with their introduction into in vitro culture were selected. The composition of the nutrient medium for the cultivation of callus tissues of S. pratensis has been optimized. A comparative analysis for the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out, and as a result it was found that extracts from callus tissue demonstrate the most pronounced antibacterial properties in concentrations of 100% and at a dilution of 1:10, in contrast to extracts from leaves and flowers of an intact plant.","PeriodicalId":20489,"journal":{"name":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Study of Antimicrobial Activity of the Callus Tissue Salvia Pratensis L. (Lamiaceae) In Vitro\",\"authors\":\"E. Maslova, Tatyana Glodik, Valeria Semykina, Natalya Gulya, T. Perelygina\",\"doi\":\"10.2991/isils-19.2019.49\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"—In this paper, we studied the antimicrobial activity of callus tissue Salvia pratensis L. obtained in vitro against Escherichia coli and Staphylococcus aureus . The most effective sterilizing agents for disinfection of plant explants with their introduction into in vitro culture were selected. The composition of the nutrient medium for the cultivation of callus tissues of S. pratensis has been optimized. A comparative analysis for the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out, and as a result it was found that extracts from callus tissue demonstrate the most pronounced antibacterial properties in concentrations of 100% and at a dilution of 1:10, in contrast to extracts from leaves and flowers of an intact plant.\",\"PeriodicalId\":20489,\"journal\":{\"name\":\"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2991/isils-19.2019.49\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 1st International Symposium Innovations in Life Sciences (ISILS 2019)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2991/isils-19.2019.49","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Study of Antimicrobial Activity of the Callus Tissue Salvia Pratensis L. (Lamiaceae) In Vitro
—In this paper, we studied the antimicrobial activity of callus tissue Salvia pratensis L. obtained in vitro against Escherichia coli and Staphylococcus aureus . The most effective sterilizing agents for disinfection of plant explants with their introduction into in vitro culture were selected. The composition of the nutrient medium for the cultivation of callus tissues of S. pratensis has been optimized. A comparative analysis for the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out, and as a result it was found that extracts from callus tissue demonstrate the most pronounced antibacterial properties in concentrations of 100% and at a dilution of 1:10, in contrast to extracts from leaves and flowers of an intact plant.