使用Corel Photo-Paint®与CMYK分裂通道进行免疫组织化学(IHC)强度分析的简单方法

Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering Pub Date : 2019-01-01 DOI:10.5220/0009587000530057

F. S. Permata, D. A. Pratama, A. Haryo

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引用次数: 0

摘要

免疫组织化学(IHC)是一种检测组织中蛋白质的常用方法。但采用ELISA法测定组织中蛋白质水平，成本较高。尽管如此，IHC颜色的强度与组织中的数量有关。因此，利用许多软件开发了用于定量测量IHC强度的图像处理。印度尼西亚常用的软件是Corel Photo-Paint®(CCP)。本文介绍了一种基于CMYK分裂通道的CCP检测免疫组化强度的简单方法。结论CCP可用于图像处理软件测量IHC强度。强度越高，灰度越少，强度越低，灰度越高

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The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel

Immunohistochemistry (IHC) is a popular method to detect protein in the tissue. But, determining protein level in tissue is using ELISA which needs more cost. Although, the intensity of IHC color relates to an amount in tissue. Therefore, image processing to measure the intensity of IHC quantitively was developed using much software. The common software used in Indonesia is Corel Photo-Paint® (CCP). This article described the simple method to measure the IHC intensity using CCP based on Cyan-Magenta-YellowBlack (CMYK) Split channel. The conclusions were CCP could be used for image processing software to measure the IHC intensity. The higher intensity has fewer grey level, the lower intensity has a higher grey level

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Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering

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