三种海参提取物对人乳腺癌细胞系的抗癌作用

Q3 Environmental Science
R. Santhanam, Nurul Shahirah Mohd Azam, Ammira Shafiqha Abdul Khadar, A. Louise, Gregory Dominic, Nur Shahida Ahmad Sofian, See Wee Han, Thiruventhan Karunakaran, Thilahgavani Nagappan, Tengku Sifzizul Tengku Muhammad3, S. Vigneswari
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引用次数: 1

摘要

海参长期以来被用来治疗各种疾病,包括抗菌、抗真菌、免疫调节和伤口愈合。首次采用MTT法和Annexin V/Propidium Iodide法比较了3种海参(Actinopyga lecanora、Holothuria atra和Stichopus vastus)提取物对癌细胞的细胞毒性。本研究探讨海参提取物对乳腺癌T-47D细胞株的保护作用。三种提取物均有明显的抑制癌细胞进展的作用,IC50值分别为6.25±0.50µg/ml (A. lecanora)、7.5±1.39µg/ml (H. atra)和3.25±0.53µg/ml (S. vastus)。采用LC-MS/MS法对提取物中的化学成分进行鉴定。测定了三种提取物的DPPH、总酚和类黄酮含量以及抗胶原酶活性。结果表明,山茱萸提取物中不含抗氧化剂和黄酮类化合物。H. atra含有酚类化合物(0.4794 mgGAE/g DW)。此外,所有试验提取物均显示出显著的抗胶原酶活性,这支持了癌细胞增殖的减少。然而,在海参的治疗特性被用于对抗乳腺癌之前,还需要对其作用机制进行更多的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticancer Potential of Three Sea Cucumber Species Extracts on Human Breast Cancer Cell Line
Sea cucumber has long been utilized as a treatment for a variety of ailments, including antibacterial, antifungal, immunomodulatory, and wound healing. As for the first time, the extracts of three sea cucumber species’ i.e, Actinopyga lecanora, Holothuria atra, and Stichopus vastus, were compared and tested on the cytotoxicity of cancer cells using MTT and Annexin V/Propidium Iodide assays. This study investigates the protection of sea cucumber extracts against the breast cancer cell line (T-47D). All three extracts were found to be actively declining the cancer cell progression, with the IC50 values of 6.25±0.50 µg/ml (A. lecanora), 7.5 ± 1.39 µg/ml (H. atra) and 3.25 ± 0.53 µg/ml (S. vastus). LC-MS/MS analysis was used to identify chemical compunds in the extracts. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic and flavonoid contents, and anti-collagenase activity were also assessed in all the three extracts. The results demonstrated the absence of antioxidant and flavonoid chemicals in S. vastus, A. leconara and H. atra extracts. However, H. atra contained phenolic compounds (0.4794 mgGAE/g DW). Furthermore, all tested extracts showed significant anti-collagenase activity, which supported the reduction of cancer cell proliferation. However, more research into the mechanism of action of the extract is needed before sea cucumbers therapeutic characteristics may be used to combat breast cancer.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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