LYMPHOCYTE ENZYMES AS AN INDICATOR OF IMMUNE RESPONSE ACTIVITY IN IXODID TICK-BORNE BORRELIOSIS

Aim of the study was to determine the pathogenetic mechanisms of the infectious and inflammatory process in the erythemal form of acute ITB based on the study of the activity of lysosomal enzymes of lymphocytes, the level of IL-2 and the clinical manifestations of the disease. Methods: The main group is represented by 609 patients hospitalized at the Krasnodar City Clinical Hospital for the period 20102019. The study group consisted of 45 patients with erythemal form of acute ITB. In the dynamics of the disease, the level of IL-2 and the cytochemical activity of acid phosphatase (AP) and nonspecific alpha-naphthyl esterase (-NAE) of lymphocytes were determined. Results: during the height of ITB, a decrease in the cytochemical activity of hydrolytic enzymes of lymphocytes was noted in comparison with the control group. IL-2 levels had a high dispersion and were associated with the clinical manifestations of the disease. A low level of IL-2 correlated with a decrease in the activity of -NAE lymphocytes. During the period of convalescence, there was a restoration of the enzymatic activity of lymphocytes, an increase in the number of cells with a pronounced activity of -NAE, typical of T-lymphocytes with killer activity. Conclusion: The hydrolytic enzymes of the lysosomes of CP and -NAE lymphocytes make it possible to judge the intensity of intracellular metabolic processes and, in combination with the clinical symptoms of the disease and the activity of IL-2, are an indicator of the state of the immune process, supplementing the results of traditional immunological studies in patients with the erythema form of acute ixodid tick-borne borreliosis. The predominance of enzymatically inactive forms of T-lymphocytes in the acute period of the disease reflects a certain deficiency of the T-cell immunity.