与hiv -结核双重感染患者和健康人相比，肺部和肺外结核患者血清来源的外泌体中玻璃连接蛋白(s蛋白)的丰度更高

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2023-05-08 DOI:10.2174/1570164620666230508140912

Ajay Vir Singh, Shweta Kushwaha, R. Yadav, Kusuma Sai Davuluri, A. Goel, Devendra Singh Chauhan

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引用次数: 0

摘要

尽管玻璃体粘连蛋白在影响某些呼吸道病原体的持久性和几种肺部疾病的进展中起着至关重要的作用，但玻璃体粘连蛋白与结核病(TB)的关系研究甚少。目前的研究旨在确定结核患者的玻璃体粘连蛋白水平是否与健康人相比有所改变。本研究纳入24例实验室确诊结核病患者(肺结核-8、肺外-8和HIV-TB双重感染-8)和8例健康个体。使用三明治酶联免疫吸附法定量检测研究参与者血清衍生外泌体中的玻璃体粘连蛋白。将检测到的玻璃体粘连素浓度与研究参与者的人口学变量以及研究组之间的变量进行比较。统计学分析采用Mann-Whitney U unpaired检验，p值< 0.05认为有统计学意义。在所有研究参与者的血清衍生外泌体中检测到玻璃体连接蛋白。人口统计学特征(性别、年龄、吸烟和饮酒习惯、咳嗽史和体重减轻)与研究参与者的玻璃体粘连素浓度无显著相关性(p值> 0.05)。肺结核患者(778.54 ng/l)和肺外结核患者(773.04 ng/l)的玻璃连接蛋白水平较高，而hiv -肺结核双感染患者(354.86 ng/l)的玻璃连接蛋白水平低于健康人(456.20ng/l)。肺结核患者的玻璃体粘连蛋白浓度(p值:0.0002)和肺外结核患者的玻璃体粘连蛋白浓度(p值:0.003)与健康对照有显著差异。本研究报道，与HIV-TB双重感染患者和健康人相比，肺部和肺外结核患者血清衍生黏着体中的玻璃体粘连蛋白浓度增加。需要进一步的研究来充分阐明较高浓度的玻璃体粘连蛋白在人结核发病过程中的诊断潜力和功能。

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Higher Abundance of Vitronectin (S-protein) in Serum-derived Exosomes of Pulmonary and Extra-Pulmonary Tuberculosis Patients as Compared to HIV-Tuberculosis Dual-infected Patients and Healthy Humans

Despite the crucial involvement of vitronectin in affecting the perseverance of certain respiratory pathogens and the progression of several lung diseases, the association of vitronectin with tuberculosis (TB) has been poorly studied. The present study aimed to determine whether vitronectin levels are altered in TB patients compared to healthy humans. Twenty-four laboratory-confirmed tuberculosis patients (pulmonary TB -8, extrapulmonary-8 and HIV-TB dual infected -8) and eight healthy individuals were included in this study. The quantitative detection of vitronectin in serum-derived exosomes of study participants was performed using a sandwich enzyme-linked immunosorbent assay. Measured concentrations of vitronectin were compared with the demographic variables of the study participants and between the study groups. The Mann–Whitney U unpaired test was used in statistical analysis, and the p-value < 0.05 was considered statistically significant. Vitronectin was detected in serum-derived exosomes of all study participants. The demographic characteristics (gender, age, smoking and alcohol consumption habit, history of cough, and weight loss) were not significantly correlated with the vitronectin concentrations of the study participants (p-value> 0.05). The level of vitronectin was higher in patients with pulmonary TB (778.54 ng/l) and extra-pulmonary-TB patients (773.04 ng/l) while lower in HIV-pulmonary TB dual-infected patients (354.86 ng/l) as compared to healthy humans (456.20ng/l). There was a significant difference between vitronectin concentrations of patients with pulmonary TB (p-value: 0.0002) and extrapulmonary TB (p-value: 0.003) compared to healthy controls. The present study reported an increased concentration of vitronectin in serum-derived exosomes of pulmonary and extra-pulmonary TB patients compared to HIV-TB dual-infected patients and healthy humans. Further studies are needed to fully elucidate the diagnostic potential and functionalities of higher concentrations of vitronectin in the pathogenic processes of human TB.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.