铜绿微囊藻提取物及其组分对人内皮细胞的剂量依赖性细胞毒性和增殖作用

IF 1.4 Q3 MARINE & FRESHWATER BIOLOGY

International Aquatic Research Pub Date : 2022-01-01 DOI:10.3153/ar22011

Seda Kuşoğlu Gültekin, Elif Mertoğlu Kamalı, K. Yılancıoğlu, N. Arda

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引用次数: 0

摘要

铜绿微囊藻(Microcystis aeruginosa)是一种危害人类和动物健康的微藻，其毒素遍布世界五大洲，据报告在257个国家的饮用水资源中存在。本研究的目的是评价铜绿假单胞菌提取物及其色谱分离组分对人内皮细胞的影响。本实验以BG-11培养基培养的铜绿假单胞菌为原料制备粗提物，并采用优化的高效液相色谱法对粗提物进行分离。采用MTT法分析海藻提取物及其6个组分对ECV304细胞的毒作用。结果显示，藻类提取物对ECV304细胞的抑制作用约为72%，高于所有馏分。毒性最大的部分是第一部分，对细胞的抑制作用为55%。除第三部分外，其余部分均有毒性，抑制率为35 ~ 40%。第三部分和一定剂量的部分对ECV304细胞有增殖活性。这些结果表明，总提取物及其组分促进或抑制细胞增殖的活性不仅与含量有关，而且与处理剂量有关。

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Dose-dependent cytotoxic and proliferative effects of Microcystis aeruginosa extract and its fractions on human endothelial cells

Microcystis aeruginosa, which spreads in five continents in the world and reported in drinking water resources in 257 countries, is a dangerous microalgae for human and animal health due to its toxins. The aim of current study was to evaluate the effects of M. aeruginosa extract and its chromatographically separated fractions on human endothelial cells. In this context, crude extract was prepared from M. aeruginosa cultured in BG-11 medium, and it was fractionated by an optimized HPLC method. Algae extract and its six fractions were then analyzed for their cytotoxic effects on ECV304 using MTT assay. The results revealed that algae extract inhibited ECV304 cells by around 72%, a higher percentage than all fractions. The most toxic fraction was the first fraction, which inhibited the cells by 55%. Other fractions, except the third one, were also toxic with 35-40% inhibition percentages. Third fraction and certain doses of some fractions showed proliferative activity on ECV304 cells. These results showed that the activities of the total extract and its fractions in promoting or inhibiting cell proliferation varied depending on not only the content but also the treatment dose.

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来源期刊

International Aquatic Research MARINE & FRESHWATER BIOLOGY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

12 weeks

期刊介绍： The journal (IAR) is an international journal that publishes original research articles, short communications, and review articles in a broad range of areas relevant to all aspects of aquatic sciences (freshwater and marine). The Journal specifically strives to increase the knowledge of most aspects of applied researches in both cultivated and wild aquatic animals in the world. The journal is fully sponsored, which means it is free of charge for authors. The journal operates a single-blind peer review process. The main research areas in aquatic sciences include: -Aquaculture- Ecology- Food science and technology- Molecular biology- Nutrition- Physiology- Water quality- Climate Change