用组氨酸脱羧酶(hdc)和16S rRNA检测金枪鱼体内组胺产菌

Q3 Environmental Science
M. Nurilmala, Novia Nanda Saputri, A. Abdullah, N. Nurjanah, R. Yusfiandayani, M. F. A. Sondita
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引用次数: 3

摘要

产生组胺的细菌可以通过分子方法来预测组胺水平的产生。本研究的目的是鉴定金枪鱼、小金枪鱼和鲣鱼(TTC)肉中产生组胺的细菌类型,并通过系统发育树构建对其进行生物信息学分析,并确定其组胺水平。采用基于hdc和16S rRNA基因的分子技术对产组胺菌进行了鉴定。用荧光光谱仪测量组胺水平。结果表明,利用特异的hdc和16S rRNA通用引物,成功鉴定出了产生组胺的细菌类型,包括莫organella morganii、hormaechei肠杆菌、Klebsiella aerogenes和bugandensis肠杆菌。系统发育树分析表明,莫氏分枝杆菌和霍马氏分枝杆菌亲缘关系较近。与此同时,其他近亲群为产气克雷伯菌、布甘肠杆菌和弗格森埃希菌。此外,冷冻金枪鱼、小金枪鱼和鲣鱼的组胺含量分别为2.96±0.22 ppm、2.14±0.23 ppm和1.02±0.97 ppm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Histamine-Producing Bacteria on Tuna Species using Histidine Decarboxylase (hdc) and 16S rRNA
Histamine-producing bacteria to predict histamine level production can be identified by a molecular approach. The purpose of this study was to identify the types of histamine-producing bacteria on tuna, little tuna, and skipjack (TTC) meat, to analyze its bioinformatics through phylogenetic tree construction also to determine the levels of its histamine. The identification of histamine-producing bacteria was conducted using a molecular technique based on the hdc and 16S rRNA genes. Histamine levels were measured by a spectrofluorometer. The results showed types of histamine-producing bacteria had been successfully identified, both using specific hdc and 16S rRNA universal primers, including Morganella morganii, Enterobacter hormaechei, Klebsiella aerogenes, and Enterobacter bugandensis. The phylogenetic tree showed that the bacteria M. morganii and E. hormaechei were closely related to one cluster. Meanwhile, the other close relative cluster were Klebsiella aerogenes, Enterobacter bugandensis, and Escherichia fergusonii. In addition, histamine levels of frozen tuna, little tuna, and skipjack were 2.96±0.22 ppm, 2.14±0.23 ppm, and 1.02±0.97 ppm, respectively.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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