{"title":"n-乙酰半胱氨酸对γ辐射诱导的人肝癌细胞毒性的影响","authors":"Heba H. Mansour , Samia A. Shouman","doi":"10.1016/j.biomag.2014.07.010","DOIUrl":null,"url":null,"abstract":"<div><p>This study investigated the effect of <em>N</em><span>-acetylcysteine (NAC) against γ-radiation-induced cytotoxicity in human hepatocellular carcinoma (HepG2) cells. HepG2 Cells were incubated with 20</span> <!-->mM of NAC for 24<!--> <!-->h prior to 6<!--> <span><span>Gy γ-irradiation. Apoptosis markers, such as caspase-3 and </span>DNA fragmentation<span> and oxidative stress markers, such as total nitrate/nitrite (NO(</span></span><em>x</em><span>)) and malondialdehyde<span><span> (MDA) levels, superoxide dismutase (SOD) and </span>glutathione (GSH) content were studied. Half of the lethal dose (LD</span></span><sub>50</sub><span>) of NAC on HepG2 cell viability was found to be 20</span> <!-->mM/mL after incubation for 48<!--> <span>h. Incubation of irradiated HepG2 cells with NAC inhibited γ-radiation-induced increases in caspase-3 activity and DNA fragmentation. Treatment with NAC before γ-radiation restored the changes induced by γ-irradiation by increasing SOD activity and GSH content in parallel with a decrease in MDA and NO(x) levels in HepG2 cells. NAC has a modulatory effect against γ-radiation-induced oxidative damage plausibly ascribable to its antioxidant/free radical scavenging ability.</span></p></div>","PeriodicalId":100181,"journal":{"name":"Biomedicine & Aging Pathology","volume":"4 4","pages":"Pages 317-321"},"PeriodicalIF":0.0000,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biomag.2014.07.010","citationCount":"1","resultStr":"{\"title\":\"Effect of N-acetylcysteine on γ-radiation-induced cytotoxicity in human hepatocellular carcinoma cells\",\"authors\":\"Heba H. Mansour , Samia A. Shouman\",\"doi\":\"10.1016/j.biomag.2014.07.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study investigated the effect of <em>N</em><span>-acetylcysteine (NAC) against γ-radiation-induced cytotoxicity in human hepatocellular carcinoma (HepG2) cells. HepG2 Cells were incubated with 20</span> <!-->mM of NAC for 24<!--> <!-->h prior to 6<!--> <span><span>Gy γ-irradiation. Apoptosis markers, such as caspase-3 and </span>DNA fragmentation<span> and oxidative stress markers, such as total nitrate/nitrite (NO(</span></span><em>x</em><span>)) and malondialdehyde<span><span> (MDA) levels, superoxide dismutase (SOD) and </span>glutathione (GSH) content were studied. Half of the lethal dose (LD</span></span><sub>50</sub><span>) of NAC on HepG2 cell viability was found to be 20</span> <!-->mM/mL after incubation for 48<!--> <span>h. Incubation of irradiated HepG2 cells with NAC inhibited γ-radiation-induced increases in caspase-3 activity and DNA fragmentation. Treatment with NAC before γ-radiation restored the changes induced by γ-irradiation by increasing SOD activity and GSH content in parallel with a decrease in MDA and NO(x) levels in HepG2 cells. NAC has a modulatory effect against γ-radiation-induced oxidative damage plausibly ascribable to its antioxidant/free radical scavenging ability.</span></p></div>\",\"PeriodicalId\":100181,\"journal\":{\"name\":\"Biomedicine & Aging Pathology\",\"volume\":\"4 4\",\"pages\":\"Pages 317-321\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.biomag.2014.07.010\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedicine & Aging Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210522014000549\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Aging Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210522014000549","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
本研究探讨了n-乙酰半胱氨酸(NAC)对γ辐射诱导的人肝癌(HepG2)细胞毒性的影响。6 Gy γ辐照前，用20 mM NAC孵育HepG2细胞24 h。研究凋亡标志物，如caspase-3和DNA断裂，氧化应激标志物，如总硝酸盐/亚硝酸盐(NO(x))和丙二醛(MDA)水平，超氧化物歧化酶(SOD)和谷胱甘肽(GSH)含量。NAC对HepG2细胞活性的半数致死剂量(LD50)为20 mM/mL。NAC可抑制γ辐射诱导的HepG2细胞caspase-3活性和DNA片段化的增加。NAC在γ辐照前处理HepG2细胞，通过提高SOD活性和GSH含量，同时降低MDA和NO(x)水平，恢复了γ辐照引起的变化。NAC对γ辐射引起的氧化损伤具有调节作用，这可能归因于其抗氧化/自由基清除能力。
Effect of N-acetylcysteine on γ-radiation-induced cytotoxicity in human hepatocellular carcinoma cells
This study investigated the effect of N-acetylcysteine (NAC) against γ-radiation-induced cytotoxicity in human hepatocellular carcinoma (HepG2) cells. HepG2 Cells were incubated with 20 mM of NAC for 24 h prior to 6 Gy γ-irradiation. Apoptosis markers, such as caspase-3 and DNA fragmentation and oxidative stress markers, such as total nitrate/nitrite (NO(x)) and malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione (GSH) content were studied. Half of the lethal dose (LD50) of NAC on HepG2 cell viability was found to be 20 mM/mL after incubation for 48 h. Incubation of irradiated HepG2 cells with NAC inhibited γ-radiation-induced increases in caspase-3 activity and DNA fragmentation. Treatment with NAC before γ-radiation restored the changes induced by γ-irradiation by increasing SOD activity and GSH content in parallel with a decrease in MDA and NO(x) levels in HepG2 cells. NAC has a modulatory effect against γ-radiation-induced oxidative damage plausibly ascribable to its antioxidant/free radical scavenging ability.
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