口服丙酮酸对模拟失重大鼠背根神经节的影响

Yuan Li, Heng Zhang, Ning-tao Ren, Chao Chen, Peng Qi, Fang-Qiang Zhou, Geng Cui
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引用次数: 2

摘要

背景:航天飞行中的长期微重力已被证明会诱发包括背根神经节(DRG)损伤在内的多器官损伤和功能障碍。本研究探讨了饮水中口服丙酮酸对后肢减重(HU)大鼠腰5 (L5) DRG组织的保护作用。方法:将sd - dawley雄性大鼠随机分为4组(N = 10):对照组(CON)、混悬液组(SUS)、生理盐水组(SAL)和丙酮酸钠组(PYR)。SUS组、SAL组和PYR组大鼠用HU模拟微重力并悬尾,持续8周。SAL组和PYR组大鼠分别饲喂生理盐水和丙酮酸盐。对L5 DRG组织进行组织病理学苏木精-伊红(H&E)染色、nissl染色神经元及髓鞘免疫荧光检查。HU后检测神经胶质细胞系源性神经营养因子(GDNF)、神经胶质纤维酸性蛋白(GFAP)、神经元特异性烯醇化酶(NSE)和S100钙结合蛋白B (S100B)的蛋白表达以及三磷酸腺苷(ATP)和三磷酸腺苷酶(ATPase)的水平。结果:数据显示,SUS组和SAL组L5 DRG神经组织结构和功能发生明显改变,而PYR组和CON组的形态学变化无显著差异;HU损伤后,SUS组和SAL组的GDNF、GFAP、NSE和S100B水平发生显著变化,而PYR组的GDNF、GFAP、NSE和S100B水平基本保持或过表达。然而,ATP和ATP酶的水平明显保持,但PYR组仍比CON组差。结论:本研究提示口服丙酮酸可有效保护大鼠L5 DRG免受HU所致的病理改变和功能障碍的损害。需要进一步调查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oral Pyruvate Effects on Dorsal Root Ganglia in Simulated Weightlessness Rats
Background: Long-term microgravity in spaceflight has been demonstrated with induction of multi-organ damage and dysfunction, including the dorsal root ganglia (DRG) injury. Present studies investigated protective effects of oral pyruvate in drinking water on lumbar 5 (L5) DRG tissues in rats subjected with hindlimb unweighting (HU). Methods: Sprague-Dawley male rats were randomly divided into four groups (N = 10): the control (CON) group, the suspension (SUS) group, the normal saline (SAL) group and the sodium pyruvate (PYR) group, respectively. Rats in the SUS, SAL and PYR groups were treated with simulated microgravity by HU with tail suspension for an 8-week period. Rats in the SAL and PYR groups were fed with normal saline and pyruvate saline, respectively. Histopathological hematoxylin-eosin (H&E) staining and Nissl-stained neurons as well as myelin sheath immunofluorescence examinations in L5 DRG tissues were carried out. The protein expression of glial cell line-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) and S100 calcium-binding protein B (S100B) as well as the levels of adenosine triphosphate (ATP) and adenosine triphosphatase (ATPase) were also determined after HU procedures. Results: Data showed that nerve tissues’ structure and function were robustly altered in L5 DRG of the SUS and SAL groups, whereas morphological changes were not significantly different between the PYR group and the CON group; levels of GDNF, GFAP, NSE and S100B were significantly changed in the SUS and SAL groups, but mostly preserved or overexpressed in group PYR following the HU injury. However, levels of ATP and ATPase were significantly retained, but still worse in the PYR group than in the CON group. The significance of oral pyruvate protection against DRG injury following the HU and the dose and formula of oral pyruvate solutions were discussed for the use of astronauts in space missions. Conclusions: This study indicates that oral pyruvate can effectively protect L5 DRG against the damage of pathological alterations and dysfunction induced by the HU in rats. Further investigations are needed.
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